Tompkins Y H, Liu G, Kim W K
Department of Poultry Science, University of GA, Athens, GA, United States.
Front Physiol. 2023 Jan 26;14:1124355. doi: 10.3389/fphys.2023.1124355. eCollection 2023.
The effects of hydrogen peroxide (HO) on the osteogenic differentiation of primary chicken mesenchymal stem cells (MSCs) were investigated. MSCs were subjected to an osteogenic program and exposed to various concentrations of HO for 14 days. Results showed that high concentrations of HO (200 and 400 nM) significantly increased pro-apoptotic marker expression and impaired osteogenic differentiation, as indicated by decreased mRNA expression levels of osteogenesis-related genes and reduced mineralization. In contrast, long-term HO exposure promoted basal expression of adipogenic markers at the expense of osteogenesis in MSCs during osteogenic differentiation, and increased intracellular reactive oxygen species (ROS) production, as well as altered antioxidant enzyme gene expression. These findings suggest that long-term HO-induced ROS production impairs osteogenic differentiation in chicken MSCs under an osteogenic program.
研究了过氧化氢（HO）对原代鸡间充质干细胞（MSCs）成骨分化的影响。将MSCs进行成骨程序处理，并暴露于不同浓度的HO中14天。结果表明，高浓度的HO（200和400 nM）显著增加促凋亡标志物表达并损害成骨分化，这表现为成骨相关基因的mRNA表达水平降低和矿化减少。相反，长期HO暴露在MSCs成骨分化过程中以牺牲成骨为代价促进了脂肪生成标志物的基础表达，并增加了细胞内活性氧（ROS）的产生，同时改变了抗氧化酶基因表达。这些发现表明，在成骨程序下，长期HO诱导的ROS产生会损害鸡MSCs的成骨分化。
