Lin C C, Kuo Y C, Huang W C, Lin C Y
Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan, Republic of China.
Chest. 1987 Dec;92(6):1022-4. doi: 10.1378/chest.92.6.1022.
A total of 50 lung cancer (CA) patients and 20 normal persons as controls were included in our study. We obtained lymphocytes from heparinized blood by Ficoll-Hypaque gradient method, and after the macrophages were removed, they were incubated with 51Cr-labelled K562 cells at concentrations of 100:1, 50:1, 12.5:1 for four hours. We calculated the cytotoxicity activity and lytic unit (LU) of natural killer (NK) cells. Significant impairment was noted in the comparison of NK cytotoxicity and LU between stage III-M1 lung CA patients (n = 28) and the control group. However, significant impairment was also noted in stage III-M1 and III-M0 patients. Four cases in stage I underwent surgery, and the NK cytotoxicity before the operation was noted to be low. After the operation, the NK cytotoxicity increased. These results indicate that NK cytotoxicity assay was impaired in stage III-M1 lung CA and also suggest that it may act as a therapeutic guide in determining the results of the operation.
我们的研究纳入了50例肺癌患者和20名正常对照者。通过Ficoll-Hypaque梯度法从肝素化血液中获取淋巴细胞，去除巨噬细胞后，将其与51Cr标记的K562细胞以100:1、50:1、12.5:1的浓度孵育4小时。我们计算了自然杀伤（NK）细胞的细胞毒性活性和裂解单位（LU）。在III-M1期肺癌患者（n = 28）与对照组之间的NK细胞毒性和LU比较中发现显著受损。然而，在III-M1期和III-M0期患者中也发现了显著受损。I期的4例患者接受了手术，术前NK细胞毒性较低。术后，NK细胞毒性增加。这些结果表明，III-M1期肺癌患者的NK细胞毒性测定受损，也表明它可能在确定手术结果方面作为一种治疗指导。
