Reproductive Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China.
Front Endocrinol (Lausanne). 2023 Jan 26;13:1072176. doi: 10.3389/fendo.2022.1072176. eCollection 2022.
The purpose of this study was to evaluate the influence of abnormal semen parameters on embryo aneuploidy based on single nucleotide polymorphism microarray (SNP array).
A total of 464 blastocysts from 103 PGT-A cycles were analyzed. The embryo quality and embryo aneuploidy rates were compared between different groups which divided by male semen parameters (sperm concentration, motility, morphology, and DFI) according the WHO criteria (2021).
The total blastocysts chromosome aneuploidy rate was 42.3% (191/452). In the teratozoospermia group, the good-quality embryo and blastocyst formation rate were lower than the normal group(44.4% vs 60.7%, P <0.01; 33.3% vs 43.5%, P <0.05), The good-quality embryo rate in normal DFI group was significantly higher than high-DFI group (59.0% vs 48.4%, P < 0.05). The blastocyst aneuploidy rate in low sperm concentration group, and high DFI group was no differences between with that in normal sperm concentration and DFI group (47.7% vs 37.8% and 44.7% vs 37.8%, P>0.05). The aneuploid rate of blastocyst in teratozoospermic and asthenozoospermia group was significantly higher than that of normal morphology and motility group (50.0% vs 34.0% and 46.7% vs 33.7%, P<0.05).
Our study revealed that sperm DFI were positively correlated with blastocyst aneuploidy rate, while sperm motility and sperm morphology rate were negatively correlated with blastocyst aneuploidy rate. Abnormal semen parameters may affect embryo quality and increase the aneuploidy rate of blastocyst chromosomes, suggesting that in clinical practice of assisted reproduction patients with abnormal semen parameters can be treated in advance to improve sperm quality, so as to reduce the impact on embryo quality and achieve a better pregnancy outcome.
本研究旨在评估基于单核苷酸多态性微阵列（SNP 微阵列）的异常精液参数对胚胎非整倍体的影响。
分析了 103 个 PGT-A 周期中的 464 个囊胚。根据世界卫生组织（2021 年）标准，将男性精液参数（精子浓度、活力、形态和 DNA 碎片指数）分为不同组，比较不同组的胚胎质量和胚胎非整倍体率。
总囊胚染色体非整倍体率为 42.3%（191/452）。在畸形精子症组中，优质胚胎和囊胚形成率低于正常组（44.4%比 60.7%，P<0.01；33.3%比 43.5%，P<0.05），正常 DFI 组的优质胚胎率显著高于高 DFI 组（59.0%比 48.4%，P<0.05）。低精子浓度组和高 DFI 组的囊胚非整倍体率与正常精子浓度和 DFI 组无差异（47.7%比 37.8%和 44.7%比 37.8%，P>0.05）。畸形精子症和弱精子症组的囊胚非整倍体率明显高于正常形态和活力组（50.0%比 34.0%和 46.7%比 33.7%，P<0.05）。
本研究表明，精子 DFI 与囊胚非整倍体率呈正相关，而精子活力和精子形态率与囊胚非整倍体率呈负相关。异常精液参数可能影响胚胎质量，增加囊胚染色体非整倍体率，提示在辅助生殖临床实践中，可对异常精液参数的患者进行提前治疗，改善精子质量，从而降低对胚胎质量的影响，达到更好的妊娠结局。
