Kini Priscilla, Wireko Solomon, Osei-Poku Priscilla, Asiedu Samuel O, Amewu Emmanuel K A, Asiedu Ebenezer, Amanor Ernest, Mensah Caleb, Wilson Mary B, Larbi Amma, Boahen Kennedy G, Sylverken Augustina A, Amato Katherine R, Kwarteng Alexander
Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.
Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana.
Health Sci Rep. 2023 Feb 7;6(2):e1104. doi: 10.1002/hsr2.1104. eCollection 2023 Feb.
Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. , a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of A gene carriage in among individuals presenting with filarial lymphedema in rural Ghana.
We recruited 160 individuals with stages I-VII lymphedema, in a cross-sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture-based techniques. The culture isolates were subjected to Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer method. A genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant.
In all, 112 . were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of , 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin-resistant haboured the A gene.
This study indicates a heightened level of methicillin-resistant isolated among filarial lymphedema patients. As a result, opportunistic infections of among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.
丝虫感染影响着热带地区超过1.5亿人。丝虫病的主要病理形式之一是淋巴水肿；在这种病症中，免疫反应会发生显著改变，导致正常菌群发生变化。[具体细菌名称]是一种人类皮肤共生菌，在免疫功能低下的个体中也可能具有致病性。因此，[具体细菌名称]在丝虫性淋巴水肿患者中可能会表现出不同的行为。为此，我们调查了加纳农村地区丝虫性淋巴水肿患者中[具体细菌名称]的抗生素耐药水平和A基因携带情况。
在加纳西部地区阿汉塔西区进行的一项横断面研究中，我们招募了160例处于I - VII期淋巴水肿的患者。使用基于标准培养的技术对来自淋巴水肿肢体溃疡、脓液和皮肤表面的拭子进行培养。培养分离株通过基质辅助激光解吸/电离飞行时间（MALDI - TOF）质谱法进行细菌鉴定。采用 Kirby - Bauer 法进行抗菌药物敏感性测试（AST）。对头孢西丁耐药的菌株通过聚合酶链反应靶向检测A基因。
总共分离出112株[具体细菌名称]。AST结果显示对氯霉素（87.5%）、四环素（83.3%）、青霉素（79.2%）和甲氧苄啶/磺胺甲恶唑（45.8%）耐药。在112株[具体细菌名称]中，51株（45.5%）对头孢西丁耐药，在对头孢西丁耐药的[具体细菌名称]中，37株（72.5%）携带A基因。
本研究表明在丝虫性淋巴水肿患者中分离出的耐甲氧西林[具体细菌名称]水平较高。因此，在加纳农村地区本就负担沉重的丝虫性淋巴水肿患者中，[具体细菌名称]的机会性感染可能会降低抗生素治疗的成功率。
