Tang Minghua, Ma Cheng, Weinheimer-Haus Eileen M, Robertson Charles E, Kofonow Jennifer M, Berman Lillian M, Waljee Akbar, Zhu Ji, Frank Daniel N, Krebs Nancy F
Section of Nutrition, Department of Pediatrics, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, United States.
Department of Statistics, University of Michigan, Ann Arbor, MI, United States.
Front Nutr. 2023 Jan 26;9:1063518. doi: 10.3389/fnut.2022.1063518. eCollection 2022.
This project aimed to evaluate the impact of meat- vs. dairy-based complementary foods on gut microbiota and whether it relates to growth.
Full-term, formula-fed infants were recruited from the metro Denver area (Colorado, US) and randomized to a meat- or dairy-based complementary diet from 5 to 12 months of age. Infant's length and weight were measured, and stool samples were collected at 5, 10, and 12 months for 16S rRNA gene sequencing and short-chain fatty acids (SCFAs) quantification.
Sixty-four infants completed the dietary intervention ( = 32/group). Weight-for-age Z (WAZ) scores increased in both groups and length-for-age Z scores (LAZ) increased in the meat group only, which led to a significant group-by-time interaction ( = 0.02) of weight-for-length Z (WLZ) score. Microbiota composition (Beta-diversity) differed between groups at 12 months (weighted PERMANOVA = 0.01) and had a group-by-time interaction of = 0.09. Microbial community richness (Chao1) increased in the meat group only. Genus had a significant group-by-time interaction and increased in the dairy group and decreased in the meat group. A significant fold change of butyric acid from 5 to 12 months was found in the meat group (+1.75, = 0.011) but not in the dairy group. Regression analysis showed that Chao1 had a negative association with WLZ and WAZ. Several genera also had significant associations with all growth Z scores.
Complementary feeding not only impacts infant growth but also affects gut microbiota maturation. Complementary food choices can affect both the gut microbiota diversity and structures and these changes in gut microbiota are associated with infant growth.
本项目旨在评估以肉类为主的辅食与以乳制品为主的辅食对肠道微生物群的影响，以及这是否与生长有关。
从美国科罗拉多州丹佛市招募足月、人工喂养的婴儿，并将其随机分为两组，从5个月至12个月分别给予以肉类为主或乳制品为主的辅食。测量婴儿的身长和体重，并在5个月、10个月和12个月时采集粪便样本，用于16S rRNA基因测序和短链脂肪酸（SCFAs）定量分析。
64名婴儿完成了饮食干预（每组32名）。两组的年龄别体重Z（WAZ）评分均升高，仅肉类组的年龄别身长Z（LAZ）评分升高，这导致身长别体重Z（WLZ）评分出现显著的组×时间交互作用（P = 0.02）。12个月时两组的微生物群组成（β多样性）不同（加权PERMANOVA，P = 0.01），组×时间交互作用P = = 0.09。仅肉类组的微生物群落丰富度（Chao1）增加。属水平上存在显著的组×时间交互作用，在乳制品组中增加，在肉类组中减少。肉类组中发现从5个月到12个月丁酸有显著的倍数变化（+1.75，P = 0.011），而乳制品组中未发现。回归分析表明，Chao1与WLZ和WAZ呈负相关。几个属也与所有生长Z评分有显著关联。
辅食喂养不仅影响婴儿生长，还会影响肠道微生物群的成熟。辅食选择会影响肠道微生物群的多样性和结构，而这些肠道微生物群的变化与婴儿生长有关。
