Yang Zhaoyan, Zong Shenfei, Jiang Guohua, Zhu Kai, Qian Ziting, Yang Kuo, Wang Zhuyuan, Cui Yiping
Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.
Anal Methods. 2023 Feb 23;15(8):1037-1046. doi: 10.1039/d2ay01855a.
All-inorganic perovskite nanocrystals (CsPbX NCs, X = Cl, Br, I) are promising fluorescence materials for biological detection due to their excellent optical properties. However, there is still a challenge to obtain stable CsPbX NCs with more biofunctions. Here, we proposed a distinct strategy by absorbing the functionalized metal nanoprobes onto the phospholipid encapsulated CsPbX NCs to achieve CsPbX-metal hybrids as probes for the detection of tumor-derived exosomes. Here, the metal nanoprobes have two functions: first, it endows phospholipid encapsulated CsPbX NCs with recognition ability; second, it avoids the fluorescence quenching of CsPbX NCs during the biological modification process by using metal nanoparticles as a bridge to connect with CsPbX NCs and various biomolecules. The obtained CPXD-AD exhibited a bright fluorescence signal, narrow full width at half-maximum (FWHM), and high specificity. Under optimal conditions, the CPXD-AD-based fluorescence-linked immunosorbent assay (FLISA) was successfully established and used for both qualitative and quantitative detection of tumor-derived exosomes.
全无机钙钛矿纳米晶体（CsPbX NCs，X = Cl、Br、I）因其优异的光学性能而成为生物检测中很有前景的荧光材料。然而，要获得具有更多生物功能的稳定CsPbX NCs仍然是一个挑战。在此，我们提出了一种独特的策略，即将功能化金属纳米探针吸附到磷脂包裹的CsPbX NCs上，以制备CsPbX-金属杂化物作为检测肿瘤衍生外泌体的探针。在此，金属纳米探针具有两个功能：第一，它赋予磷脂包裹的CsPbX NCs识别能力；第二，通过使用金属纳米颗粒作为桥梁连接CsPbX NCs和各种生物分子，避免了CsPbX NCs在生物修饰过程中的荧光猝灭。所获得的CPXD-AD表现出明亮的荧光信号、窄的半高宽（FWHM）和高特异性。在最佳条件下，成功建立了基于CPXD-AD的荧光酶联免疫吸附测定法（FLISA），并用于肿瘤衍生外泌体的定性和定量检测。
