Advanced Photonics Center , Southeast University , Nanjing 210096 , China.
ACS Appl Mater Interfaces. 2019 Dec 26;11(51):47671-47679. doi: 10.1021/acsami.9b12924. Epub 2019 Oct 30.
Lead halide perovskite nanocrystals (NCs) are promising optical materials in many fields. However, their poor moisture stability, significant toxicity, and difficulty to be further functionalized greatly hinder their applications in bioimaging. Here, a universal strategy is demonstrated by simply encapsulating CsPbX (X = Cl, Br, I) NCs into phospholipids to achieve CsPbX-phospholipid micelles (CsPbX@phospholipid) as probes for multiplex encoding cellular imaging or tumor-targeted imaging. The layer of phospholipids endows CsPbX NCs with superior water-resistant characteristics, the ability to be further biofunctionalized, and greatly improved biocompatibility. The CsPbX@phospholipid micelles exhibited strong luminescence with narrow fwhm in water for more than four months. Specifically, even after being modified with folic acid, the bright fluorescence of the micelles was well retained, which were employed for the targeting of Hela cells. Finally, the greatly reduced toxicity of the CsPbX@phospholipid micelles was verified using HeLa cells and zebrafish as in vitro and in vivo models, respectively.
卤化铅钙钛矿纳米晶体（NCs）在许多领域都是很有前途的光学材料。然而，它们较差的耐湿性、显著的毒性以及难以进一步功能化极大地阻碍了它们在生物成像中的应用。在这里，通过简单地将 CsPbX（X = Cl、Br、I）NCs 封装在磷脂中，展示了一种通用策略，从而实现了 CsPbX-磷脂胶束（CsPbX@phospholipid）作为用于多重编码细胞成像或肿瘤靶向成像的探针。磷脂层赋予 CsPbX NCs 优异的耐水性、进一步生物功能化的能力和大大提高的生物相容性。CsPbX@phospholipid 胶束在水中具有超过四个月的强发光和窄半峰全宽（fwhm）。具体来说，即使经过叶酸修饰，胶束的明亮荧光也能很好地保留下来，可用于 Hela 细胞的靶向。最后，使用 HeLa 细胞和斑马鱼分别作为体外和体内模型，验证了 CsPbX@phospholipid 胶束的毒性大大降低。
