Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Environ Sci Technol. 2023 Feb 21;57(7):2887-2897. doi: 10.1021/acs.est.3c00032. Epub 2023 Feb 13.
A novel brominated flame retardant decabromodiphenyl ethane (DBDPE) has become a ubiquitous emerging pollutant; hence, the knowledge of its long-term toxic effects and underlying mechanism would be critical for further health risk assessment. In the present study, the multi- and transgenerational toxicity of DBDPE was investigated in zebrafish upon a life cycle exposure at environmentally relevant concentrations. The significantly increased malformation rate and declined survival rate specifically occurred in unexposed F2 larvae suggested transgenerational development toxicity by DBDPE. The changing profiles revealed by transcriptome and DNA methylome confirmed an increased susceptibility in F2 larvae and figured out potential disruptions of glycolipid metabolism, mitochondrial energy metabolism, and neurodevelopment. The changes of biochemical indicators such as ATP production confirmed a disturbance in the energy metabolism, whereas the alterations of neurotransmitter contents and light-dark stimulated behavior provided further evidence for multi- and transgenerational neurotoxicity in zebrafish. Our findings also highlighted the necessity for considering the long-term impacts when evaluating the health of wild animals as well as human beings by emerging pollutants.
一种新型溴化阻燃剂十溴二苯乙烷（DBDPE）已成为一种无处不在的新兴污染物；因此，了解其长期的毒性效应和潜在机制对于进一步的健康风险评估至关重要。在本研究中，通过在环境相关浓度下进行生命周期暴露，研究了 DBDPE 对斑马鱼的多代和跨代毒性。未暴露的 F2 幼虫中畸形率显著增加和存活率下降表明 DBDPE 具有跨代发育毒性。转录组和 DNA 甲基组揭示的变化图谱证实了 F2 幼虫的易感性增加，并指出糖脂代谢、线粒体能量代谢和神经发育的潜在破坏。生化指标如 ATP 产生的变化证实了能量代谢的紊乱，而神经递质含量和明暗刺激行为的改变为斑马鱼的多代和跨代神经毒性提供了进一步的证据。我们的研究结果还强调了在评估野生动物和人类健康时，有必要考虑到新兴污染物的长期影响。
