Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China; State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China.
State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China.
J Hazard Mater. 2024 Mar 5;465:133228. doi: 10.1016/j.jhazmat.2023.133228. Epub 2023 Dec 14.
The brominated flame retardant decabromodiphenyl ethane (DBDPE) has been extensively used following restrictions on BDE-209 and thus, been frequently detected in aquatic environment. However, information on impact of DBDPE on fish development and the potential mechanisms remains scarce. In present study, developing zebrafish were employed as a study model. Embryos were exposed until 5 d to DBDPE at concentrations of 0, 3, 30, and 300 μg/L, following which the impact on larval development was investigated. DBDPE bioaccumulation and locomotor hyperactivity were observed in developing zebrafish exposed to DBDPE. Transcriptome and bioinformatics analyses indicated that pathways associated with cardiac muscle contraction and retinol metabolism were notably affected. The mechanisms of DBDPE to induce locomotor abnormality were further investigated by analyzing levels of retinol and retinol metabolites, eye and heart histology, heart rates, and ATPase activity. Our results indicate that locomotor hyperactivity observed in larvae exposed to DBDPE results from abnormal heartbeat, which in turn is attributable to inhibition of Na/K-ATPase activity. Furthermore, DBDPE did not change larval eye histology and contents of retinoid (retinol, retinal, and retinoic acid). This study provides insight into the mechanisms underlying DBDPE-induced developmental toxicity and highlights the need for addressing the environmental risks for aquatic organisms.
溴代阻燃剂十溴二苯乙烷（DBDPE）在限制使用 BDE-209 后被广泛使用，因此在水生环境中频繁被检出。然而，DBDPE 对鱼类发育的影响及其潜在机制的信息仍然很少。在本研究中，使用发育中的斑马鱼作为研究模型。胚胎在 0、3、30 和 300μg/L 的 DBDPE 浓度下暴露至 5 天，然后研究其对幼鱼发育的影响。暴露于 DBDPE 的发育中的斑马鱼出现 DBDPE 生物积累和运动过度活跃的现象。转录组和生物信息学分析表明，与心肌收缩和视黄醇代谢相关的途径受到明显影响。通过分析视黄醇和视黄醇代谢物水平、眼睛和心脏组织学、心率和 ATP 酶活性，进一步研究了 DBDPE 诱导运动异常的机制。我们的研究结果表明，暴露于 DBDPE 的幼鱼出现运动过度活跃的现象是由于心跳异常引起的，而心跳异常是由于 Na/K-ATP 酶活性受到抑制所致。此外，DBDPE 没有改变幼鱼的眼睛组织学和视黄醇（视黄醇、视网膜和视黄酸）含量。本研究深入了解了 DBDPE 引起的发育毒性的机制，并强调了需要解决对水生生物的环境风险。
