Department of Nephrology, 74720Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Department of Nephropathy and Rheumatology, Changshun County Medical Group Central Hospital, Changshun, China.
Hum Exp Toxicol. 2023 Jan-Dec;42:9603271221145386. doi: 10.1177/09603271221145386.
The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis. Albiflorin (AF) is an effective composition extracted from Paeonia Alba Radix and has been administrated for various diseases. Nevertheless, there is no research reporting the effect of AF on MPGN.Our work aims to probe into the role and possible mechanism of AF on MPGN.We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis and and identified the related signaling pathways. human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.SPSS 18.0 was used to analyze the data. AF attenuated the proliferation and inflammation both and . In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment. The fibrosis of LPS-treated HMCs and MPGN rats was also reduced by AF. Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats. Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist. AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
在中国，最常见的肾小球肾炎类型是系膜增生性肾小球肾炎（MPGN），其特征为系膜细胞过度增殖和炎症以及纤维化。白芍苷（AF）是从白芍中提取的一种有效成分，已用于治疗各种疾病。然而，目前尚无研究报道 AF 对 MPGN 的影响。我们的工作旨在探讨 AF 对 MPGN 的作用及可能的机制。我们研究了 AF 对系膜细胞过度增殖、炎症和纤维化的影响，并确定了相关的信号通路。人系膜细胞（HMCs）和雄性 Sprague Dawley（SD）大鼠。使用 SPSS 18.0 分析数据。AF 减轻了增殖和炎症。具体来说，AF 降低了脂多糖（LPS）处理的 HMCs 和 MPGN 大鼠中的 ki67 表达，并且 AF 处理后炎症细胞因子的 mRNA 表达或含量降低。AF 还减轻了 LPS 处理的 HMCs 和 MPGN 大鼠的纤维化。此外，AF 有效抑制了 LPS 处理的 HMCs 和 MPGN 大鼠的 24 小时尿蛋白，改善了肾功能，并减轻了 MPGN 大鼠的血脂异常和病理损伤。此外，我们发现 AF 的保护作用伴随着 PI3K/AKT/NF-κB 通路的阻断，并且 IGF-1（PI3K 激动剂）可逆转 AF 对 MPGN 的抑制作用。AF 通过 PI3K/AKT/NF-κB 信号通路减轻 MPGN，从而抑制系膜细胞过度增殖、炎症和纤维化。
