血清骨重塑参数和转录组谱分析揭示了与蛋鸡龙骨骨折相关的异常骨代谢。

Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens.

机构信息

College of Life Science, Northeast Agricultural University, 150030 Harbin, China.

College of Animal Science and Technology, Northeast Agricultural University, 150030 Harbin, China.

出版信息

Poult Sci. 2023 Apr;102(4):102438. doi: 10.1016/j.psj.2022.102438. Epub 2022 Dec 19.

DOI:10.1016/j.psj.2022.102438

PMID:36780704

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9947423/

Abstract

Keel bone fractures affect welfare, health, and production performance in laying hens. A total of one hundred and twenty 35-wk-old Hy-line Brown laying hens with normal keel (NK) bone were housed in furnished cages and studied for ten weeks to investigate the underlying mechanism of keel bone fractures. At 45 wk of age, the keel bone state of birds was assessed by palpation and X-ray, and laying hens were recognized as NK and fractured keel (FK) birds according to the presence or absence of fractures in keel bone. The serum samples of 10 NK and 10 FK birds were collected to determine bone metabolism-related indexes and slaughtered to collect keel bones for RNA-sequencing (RNA-seq), Micro-CT, and histopathological staining analyses. The results showed that the concentrations of Ca, phosphorus, calcitonin, 25-hydroxyvitamin D, and osteocalcin and activities of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP) in serum samples of FK birds were lower than those of NK birds (P < 0.05), but the concentrations of parathyroid hormone, osteoprotegerin, and corticosterone in serum samples of FK birds were higher than those of NK birds (P < 0.05). TRAP staining displayed that FK bone increased the number of osteoclasts (P < 0.05). Micro-CT analysis indicated that FK bone decreased bone mineral density (P < 0.05). Transcriptome sequencing analysis of NK and FK bones identified 214 differentially expressed genes (DEGs) (|log2FoldChange| > 1, P < 0.05), among which 88 were upregulated and 126 downregulated. Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) analysis indicated that 14 DEGs related to skeletal muscle movement and bone Ca transport (COL6A1, COL6A2, COL6A3, PDGFA, MYLK2, EGF, CAV3, ADRA1D, BDKRB1, CACNA1S, TNN, TNNC1, TNNC2, and RYR3) were enriched in focal adhesion and Ca signaling pathway, regulating bone quality. This study suggests that abnormal bone metabolism related to keel bone fractures is possibly responded to fracture healing in laying hens.

摘要

龙骨骨折会影响蛋鸡的福利、健康和生产性能。本研究选择 120 只 35 周龄的海兰褐蛋鸡，在笼养条件下饲养 10 周，研究龙骨骨折的潜在机制。45 周龄时，通过触诊和 X 射线评估鸡的龙骨状况，并根据龙骨是否骨折将鸡分为正常龙骨（NK）组和骨折龙骨（FK）组。采集 10 只 NK 组和 10 只 FK 组鸡的血清样本，测定与骨代谢相关的指标，屠宰后采集龙骨进行 RNA 测序（RNA-seq）、Micro-CT 和组织病理学染色分析。结果表明，FK 组鸡血清中 Ca、P、降钙素、25-羟维生素 D、骨钙素和碱性磷酸酶、抗酒石酸酸性磷酸酶（TRAP）活性均低于 NK 组（P < 0.05），而 FK 组鸡血清中甲状旁腺激素、骨保护素和皮质酮浓度高于 NK 组（P < 0.05）。TRAP 染色显示 FK 骨增加了破骨细胞数量（P < 0.05）。Micro-CT 分析表明 FK 骨降低了骨密度（P < 0.05）。对 NK 和 FK 骨的转录组测序分析共鉴定出 214 个差异表达基因（DEGs）（|log2FoldChange| > 1，P < 0.05），其中 88 个上调，126 个下调。KEGG 通路分析表明，COL6A1、COL6A2、COL6A3、PDGFA、MYLK2、EGF、CAV3、ADRA1D、BDKRB1、CACNA1S、TNN、TNNC1、TNNC2 和 RYR3 等 14 个与骨骼肌肉运动和骨钙转运相关的 DEGs 在粘着斑和钙信号通路中富集，调节骨质量。本研究表明，与龙骨骨折相关的异常骨代谢可能是蛋鸡骨折愈合的反应。