State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu, China.
Plant Signal Behav. 2023 Dec 31;18(1):2178085. doi: 10.1080/15592324.2023.2178085.
In acid soil, aluminum (Al) toxicity is one of the main factors limiting agricultural output. As is known to all, the cell wall is the first line of defense against metals that serves as a significant target of Al toxicity and also is crucial for Al detoxification. However, nothing is known about how this process is transcriptionally regulated. Here, we describe recent findings to understand the role of two kinds of transcription factors in regulating the cell wall composition and modification in response to Al stress in encodes a NAM, ATAF1/2, and cup-shaped cotyledon 2 (NAC) transcription factor, loss function of enhanced Al tolerance with the decreased Al content and xyloglucan content in the cell wall. Next, we characterized one xyloglucan endotransglucosylase/hydrolase (XTH), XTH31, which is previously reported to participate in Al stress, acted downstream of ANAC017 to regulate Al tolerance in . In addition, we also identified MYB103, an R2R3-type transcription factor. disruption caused Al sensitivity, and mutants' xyloglucan had a high -acetylation level. Additionally, it was discovered that TRICHOME BIREFRINGENCE-LIKE27 (TBL27), which is in charge of xyloglucan's -acetylation, functions downstream of MYB103 through the direct binding of the MYB103 to the promoter of the TBL27 to influence Arabidopsis's sensitivity to Al. In summary, our research showed that two distinct molecular modules modulate Arabidopsis cell wall composition and modification to positively influence Al resistance.
在酸性土壤中，铝（Al）毒性是限制农业产量的主要因素之一。众所周知，细胞壁是抵御金属的第一道防线，是 Al 毒性的重要靶标，也是 Al 解毒的关键。然而，人们对这个过程如何被转录调控还一无所知。在这里，我们描述了最近的发现，以了解两种转录因子在调节细胞壁组成和修饰以响应 Al 胁迫中的作用。 编码一种 NAM、ATAF1/2 和杯状子叶 2（NAC）转录因子， 功能丧失增强了 Al 耐受性，同时细胞壁中的 Al 含量和木葡聚糖含量降低。接下来，我们对一个 previously reported to participate in Al stress, acted downstream of ANAC017 to regulate Al tolerance in 。此外，我们还鉴定了 MYB103，一种 R2R3 型转录因子。 突变体对 Al 敏感，突变体的木葡聚糖乙酰化水平较高。此外，还发现负责木葡聚糖 - 乙酰化的 TRICHOME BIREFRINGENCE-LIKE27（TBL27）通过 MYB103 与 TBL27 启动子的直接结合在 MYB103 下游发挥作用，影响拟南芥对 Al 的敏感性。总之，我们的研究表明，两个不同的分子模块调节拟南芥细胞壁组成和修饰，以积极影响 Al 抗性。
