School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
Int J Mol Sci. 2023 Oct 10;24(20):15052. doi: 10.3390/ijms242015052.
Cadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This study described the Cd-induced gene from the hyperaccumulator . Its subcellular localization showed that ApHIPP26 was located in the nucleus. Transgenic overexpressing exhibited a significant increase in main root length and fresh weight under Cd stress. Compared with wild-type lines, Cd accumulated much more in transgenic both aboveground and underground. Under Cd stress, the expression of genes related to the absorption and transport of heavy metals underwent different changes in parallel, which were involved in the accumulation and distribution of Cd in plants, such as and . Under Cd stress, the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) in the transgenic lines were higher than those in the wild type. The physiological and biochemical indices showed that the proline and chlorophyll contents in the transgenic lines increased significantly after Cd treatment, while the malondialdehyde (MDA) content decreased. In addition, the gene expression profile analysis showed that improved the tolerance of to Cd by regulating the changes of related genes in plant hormone signal transduction pathway. In conclusion, plays an important role in cadmium tolerance by alleviating oxidative stress and regulating plant hormones, which provides a basis for understanding the molecular mechanism of cadmium tolerance in plants and provides new insights for phytoremediation in Cd-contaminated areas.
镉（Cd）是一种有毒重金属，在植物中积累后会严重影响新陈代谢，而且还会通过食物链对人类造成不良影响。HIPP 基因家族因其特殊的结构域和分子结构而被证明对 Cd 胁迫具有高度耐受性。本研究描述了超积累植物 中的 Cd 诱导基因 。其亚细胞定位表明 ApHIPP26 位于细胞核中。过表达 基因的 转基因植株在 Cd 胁迫下主根长度和鲜重显著增加。与野生型相比，转基因 地上部和地下部 Cd 积累量均明显增加。在 Cd 胁迫下，与重金属吸收和转运相关的基因表达平行发生不同变化，参与 Cd 在植物中的积累和分布，如 和 。在 Cd 胁迫下，转基因株系中抗氧化酶（超氧化物歧化酶、过氧化物酶、过氧化氢酶和抗坏血酸过氧化物酶）的活性高于野生型。生理生化指标表明，转基因株系在 Cd 处理后脯氨酸和叶绿素含量显著增加，而丙二醛（MDA）含量降低。此外，基因表达谱分析表明， 通过调节植物激素信号转导途径中相关基因的变化，提高了 对 Cd 的耐受性。综上所述， 通过缓解氧化应激和调节植物激素，在 Cd 耐受中发挥重要作用，为理解植物 Cd 耐受的分子机制提供了依据，并为 Cd 污染区的植物修复提供了新的思路。
