Rotwein P, Pollock K M, Watson M, Milbrandt J D
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Endocrinology. 1987 Dec;121(6):2141-4. doi: 10.1210/endo-121-6-2141.
The function of insulin-like growth factors I and II (IGF-I and IGF-II) in embryogenesis is unknown. To investigate the ontogeny of IGF gene expression during mammalian development we used a highly sensitive and specific solution-hybridization assay to determine the steady state levels of IGF mRNAs during midgestation in the rat. IGF-I mRNA can be detected as early as day 11 of embryonic development and rises 8.6-fold over the ensuing 48 h. By contrast IGF-II mRNA is relatively constant over days 11-14 of gestation. These observations suggest that both IGFs may play important roles in early fetal development.
胰岛素样生长因子I和II（IGF-I和IGF-II）在胚胎发生中的功能尚不清楚。为了研究哺乳动物发育过程中IGF基因表达的个体发生，我们使用了一种高度灵敏且特异的溶液杂交分析法，以确定大鼠妊娠中期IGF mRNA的稳态水平。早在胚胎发育的第11天就能检测到IGF-I mRNA，在随后的48小时内其水平上升了8.6倍。相比之下，IGF-II mRNA在妊娠第11 - 14天相对恒定。这些观察结果表明，两种IGF可能在胎儿早期发育中发挥重要作用。
