聚合酶链反应等位基因竞争延伸（PCR Allele competitive Extension，PACE）。

PCR Allele Competitive Extension (PACE).

机构信息

Julius Kuehn-Institute (JKI), Institute for Breeding Research on Horticultural Crops, Quedlinburg, Germany.

出版信息

Methods Mol Biol. 2023;2638:263-271. doi: 10.1007/978-1-0716-3024-2_18.

Abstract

PCR allele competitive extension (PACE™) is a marker system to analyze single nucleotide polymorphisms (SNPs). As strongly related technologies like kompetitive allele-specific PCR (KASP™) or Amplifluor, PACE is based on a polymerase chain reaction (PCR) with two competing allele-specific primers and an endpoint fluorescent measurement. The system requires sequence information surrounding the SNP of interest and a qPCR machine or a fluorescent plate reader.

摘要

PCR 等位基因竞争延伸 (PACE™) 是一种用于分析单核苷酸多态性 (SNP) 的标记系统。与类似的竞争等位基因特异性 PCR (KASP™) 或 Amplifluor 等技术一样，PACE 基于带有两个竞争等位基因特异性引物的聚合酶链反应 (PCR) 和终点荧光测量。该系统需要感兴趣 SNP 周围的序列信息，以及一台 qPCR 仪器或荧光板读数仪。