Kannourakis G, Bol S
Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
Exp Hematol. 1987 Dec;15(11):1103-8.
Depletion of adherent cells, followed by simultaneous immunomagnetic bead depletion of Leu 4+, Leu 7+, Leu 11+, Leu M1+, Leu M3+, B1+, WEM-G11+, and Glycophorin A+ cells from normal bone marrow mononuclear cells, consistently led to recoveries of erythroid and nonerythroid colony-forming cells of greater than 100% and enrichment of 13- to 99-fold. Similarly, the recovery of mixed erythroid colony-forming cells was greater than 100%, with enrichments of 7.5- to 262-fold. This simple procedure, when used on normal bone marrow and beta-thalassemic peripheral blood mononuclear cells, as well as providing significant enrichment, suggests that colony assays on unfractionated human mononuclear cells specifically underestimate the number of BFU-E, Mix-CFC, and nonerythroid-CFC present.
从正常骨髓单个核细胞中去除贴壁细胞，随后同时用免疫磁珠去除Leu 4 +、Leu 7 +、Leu 11 +、Leu M1 +、Leu M3 +、B1 +、WEM - G11 +和血型糖蛋白A +细胞，始终能使红系和非红系集落形成细胞的回收率超过100%，富集倍数为13至99倍。同样，混合红系集落形成细胞的回收率大于100%，富集倍数为7.5至262倍。这一简单程序用于正常骨髓和β地中海贫血外周血单个核细胞时，除了能提供显著富集外，还表明对未分级的人单个核细胞进行集落测定会特异性低估存在的BFU - E、Mix - CFC和非红系CFC的数量。
