疏水簇分析：一种比较和分析氨基酸序列的有效新方法。

Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences.

作者信息

Gaboriaud C, Bissery V, Benchetrit T, Mornon J P

机构信息

Groupe Cristallographie et Simulations Interactives des Macromolécules Biologiques, CNRS UA09, Université, Paris, France.

出版信息

FEBS Lett. 1987 Nov 16;224(1):149-55. doi: 10.1016/0014-5793(87)80439-8.

DOI:10.1016/0014-5793(87)80439-8

PMID:3678489

Abstract

A new method for comparing and aligning protein sequences is described. This method, hydrophobic cluster analysis (HCA), relies upon a two-dimensional (2D) representation of the sequences. Hydrophobic clusters are determined in this 2D pattern and then used for the sequence comparisons. The method does not require powerful computer resources and can deal with distantly related proteins, even if no 3D data are available. This is illustrated in the present report by a comparison of human haemoglobin with leghaemoglobin, a comparison of the two domains of liver rhodanese (thiosulphate sulphurtransferase) and a comparison of plastocyanin and azurin.

摘要

本文描述了一种用于比较和比对蛋白质序列的新方法。这种方法，即疏水簇分析（HCA），依赖于序列的二维（2D）表示。在这种二维模式中确定疏水簇，然后将其用于序列比较。该方法不需要强大的计算机资源，并且即使没有三维数据也能处理远缘相关的蛋白质。本报告通过将人血红蛋白与豆血红蛋白进行比较、肝硫氰酸酶（硫代硫酸盐硫转移酶）的两个结构域进行比较以及质体蓝素和天青蛋白进行比较来说明这一点。