Institute of Ecology and Earth Sciences, University of Tartu, 2 J. Liivi Street, 50409, Tartu, Estonia.
Institute of Botany of the Czech Academy of Sciences, Zámek 1, 252 43, Průhonice, Czech Republic.
Mycorrhiza. 2023 Jun;33(3):211-220. doi: 10.1007/s00572-023-01105-9. Epub 2023 Feb 14.
The use of arbuscular mycorrhizal (AM) fungal inoculants as a means to promote plant growth is gaining momentum worldwide. Although there is an increasing number of commercial products available for various applications, the quality of these remains uncertain. We determined the AM fungal species composition in eleven inoculants from four producers by using DNA metabarcoding and compared them to the AM fungal species declared on the product labels. Our DNA metabarcoding of the inoculants revealed a concerning discrepancy between the declared and detected AM fungal species compositions of the products. While nine products contained at least one declared species, two did not contain any matching species and all inoculants but one contained additional species not declared on the product label. These findings highlight the need for better guidelines and industry standards to ensure consumer protection in the AM fungal inoculum market. Additionally, we call for caution when using commercial AM fungal inoculants in scientific experiments without confirmatory information about their species composition.
菌根真菌(AM)接种剂作为促进植物生长的手段在全球范围内得到了越来越多的应用。尽管有越来越多的商业产品可用于各种应用,但这些产品的质量仍然不确定。我们通过 DNA 宏条形码技术确定了来自四个生产商的十一种接种剂中的 AM 真菌物种组成,并将其与产品标签上声明的 AM 真菌物种进行了比较。我们对接种剂的 DNA 宏条形码分析显示,产品声明的和检测到的 AM 真菌物种组成之间存在令人担忧的差异。虽然九个产品至少含有一种声明的物种,但有两个产品没有任何匹配的物种,所有接种剂(除一个外)都含有产品标签上未声明的其他物种。这些发现强调了需要更好的指南和行业标准来确保 AM 真菌接种剂市场的消费者保护。此外,我们呼吁在没有关于其物种组成的确认信息的情况下,在科学实验中使用商业 AM 真菌接种剂时要谨慎。