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人诱导多能干细胞的确定内胚层和肝细胞分化数据集。

A dataset of definitive endoderm and hepatocyte differentiations from human induced pluripotent stem cells.

机构信息

RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, 230-0045, Japan.

Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, 230-0045, Japan.

出版信息

Sci Data. 2023 Feb 14;10(1):93. doi: 10.1038/s41597-023-02001-9.

DOI:10.1038/s41597-023-02001-9
PMID:36788249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9929325/
Abstract

Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation time-course of hepatocyte-like cells. We performed methylome and transcriptome analyses for hepatocyte-like cell differentiation. We also analyzed DE-like cell differentiation by methylome, transcriptome, chromatin accessibility, and GATA6 binding profiles, using finer time-course samples. In this manuscript, we provide a detailed description of the dataset and the technical validations. Our data may be valuable for the analysis of the molecular mechanisms underlying hepatocyte and DE differentiations.

摘要

肝细胞是肝脏的主要实质细胞类型，在肝脏功能中发挥着重要作用。肝细胞样细胞可以通过诱导多能干细胞（iPSCs）体外分化为确定内胚层（DE）样细胞和肝前体细胞样细胞。在这里，我们探索了肝细胞样细胞体外分化的时间过程。我们进行了肝细胞样细胞分化的甲基化组和转录组分析。我们还使用更精细的时间过程样本，通过甲基化组、转录组、染色质可及性和 GATA6 结合谱分析，研究了 DE 样细胞的分化。在本手稿中，我们提供了数据集的详细描述和技术验证。我们的数据对于分析肝细胞和 DE 分化的分子机制可能具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/d68f423c4392/41597_2023_2001_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/ba66f968a647/41597_2023_2001_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/75d4112d2e55/41597_2023_2001_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/9128b33696de/41597_2023_2001_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/2d4e6c8cf904/41597_2023_2001_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/72823dd638f8/41597_2023_2001_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/d68f423c4392/41597_2023_2001_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/ba66f968a647/41597_2023_2001_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/75d4112d2e55/41597_2023_2001_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/9128b33696de/41597_2023_2001_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/2d4e6c8cf904/41597_2023_2001_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/72823dd638f8/41597_2023_2001_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0729/9929325/d68f423c4392/41597_2023_2001_Fig6_HTML.jpg

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本文引用的文献

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GATA6 is predicted to regulate DNA methylation in an in vitro model of human hepatocyte differentiation.在人类肝细胞分化的体外模型中，预测GATA6可调节DNA甲基化。
Commun Biol. 2022 May 4;5(1):414. doi: 10.1038/s42003-022-03365-1.
2
GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells.GATA6在人类确定内胚层的诱导、胰腺发育及胰腺β细胞功能中发挥重要作用。
Stem Cell Reports. 2017 Mar 14;8(3):589-604. doi: 10.1016/j.stemcr.2016.12.026. Epub 2017 Feb 9.
3
One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells.
一种标准化分化程序可稳健地从大量人类多能干细胞中生成具有代谢多样性的均匀肝细胞培养物。
Stem Cell Rev Rep. 2016 Feb;12(1):90-104. doi: 10.1007/s12015-015-9621-9.
4
MOIRAI: a compact workflow system for CAGE analysis.MOIRAI：CAGE 分析的紧凑型工作流系统。
BMC Bioinformatics. 2014 May 16;15:144. doi: 10.1186/1471-2105-15-144.
5
Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position.天然染色质易位用于快速灵敏的染色质开放性、DNA 结合蛋白和核小体位置的表观基因组分析。
Nat Methods. 2013 Dec;10(12):1213-8. doi: 10.1038/nmeth.2688. Epub 2013 Oct 6.
6
Production of hepatocyte-like cells from human pluripotent stem cells.人多能干细胞向肝样细胞的分化。
Nat Protoc. 2013 Feb;8(2):430-7. doi: 10.1038/nprot.2012.153.
7
Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells: application to liver-specific functional and cytotoxic assays.从人多能干细胞中无饲养层和无血清培养肝细胞、胆管细胞及其增殖祖细胞：在肝脏特异性功能和细胞毒性检测中的应用
Cell Reprogram. 2012 Apr;14(2):171-85. doi: 10.1089/cell.2011.0064. Epub 2012 Mar 2.
8
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9
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BMC Bioinformatics. 2010 Nov 30;11:587. doi: 10.1186/1471-2105-11-587.
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