Department of Chemistry, Federal University of Espírito Santo, Vitória-ES, Brazil.
Department of Pharmaceutical Sciences, Federal University of Espírito Santo, Vitoria-ES, Brazil.
Nat Prod Res. 2023 Nov-Dec;37(24):4267-4273. doi: 10.1080/14786419.2023.2179623. Epub 2023 Feb 14.
Ethanol extract from the aerial parts of L. as well as the latex of the plant suspended in water are used by the Brazilian population for the treatment of various diseases, including cancer. The purposes of this study were to determine if the ethanol extract is effective as cytotoxic agent against gastric adenocarcinoma cells (AGS) and its chemical composition by GC-MS, ESI-(-)-FT-ICR MS and (-)-ESI-LTQ-MS/MS. The results were compared with that of latex previously described by us. Hexane and aqueous fractions showed higher cytotoxicity on AGS cells. Nine triterpene compounds were detected by GC-MS in hexane fraction, including euphol and friedelin, while ellagic acid was identified as main phenolic compound in aqueous extract. Therefore, the greater cytotoxic activity of the ethanol extract of the aerial parts of for gastric cancer, when compared to latex, seems to originate from the antiproliferative effects of ellagic acid and triterpenes.
从 地上部分提取的乙醇提取物和悬浮在水中的植物乳胶被巴西民众用于治疗多种疾病，包括癌症。本研究的目的是确定乙醇提取物是否对胃腺癌细胞（AGS）具有细胞毒性作用，并通过 GC-MS、ESI-(-)-FT-ICR MS 和 (-)-ESI-LTQ-MS/MS 分析其化学成分。将结果与我们之前描述的乳胶进行比较。正己烷和水相部分对 AGS 细胞显示出更高的细胞毒性。通过 GC-MS 在正己烷部分检测到 9 种三萜类化合物，包括 euphol 和 friedelin，而鞣花酸被鉴定为水提取物中的主要酚类化合物。因此，与乳胶相比，地上部分的乙醇提取物对胃癌的更高细胞毒性活性似乎源于鞣花酸和三萜类化合物的抗增殖作用。
