Mousavinafchi Seyedeh Bita, Rahimi Ebrahim, Shakerian Amir
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch Islamic Azad University Shahrekord Iran.
Research Center of Nutrition and Organic Products, Shahrekord Branch Islamic Azad University Shahrekord Iran.
Food Sci Nutr. 2022 Nov 21;11(2):1142-1153. doi: 10.1002/fsn3.3152. eCollection 2023 Feb.
spp. genera is one of the most common causes of microbial enteritis worldwide. The objective of this work was to investigate the antimicrobial resistance (AMR) patterns, virulence genes, and genetic variation of thermophilic species collected from chicken meat samples in Iran. A total of 255 meat specimens were taken and transferred to the laboratory. Culture methods were utilized to identify the genus, and PCR and sequencing were performed to confirm the organisms. Antimicrobial susceptibility evaluation was performed using broth microdilution for six antimicrobials [ciprofloxacin (CIP), nalidixic acid (NAL), sitafloxacin (SIT), erythromycin (ERY), tetracycline (TET), and gentamicin (GEN)]. By using PCR, AMR and virulence genes were detected. The detection rate of spp. was 64 (25.09%) out of 255 meat samples, with and accounting for 41 (64.06%) and 14 (21.87%), respectively. Other isolates accounted for 14.06% of the total (nine samples). The antibiotic susceptibility of all isolates was tested using six antibiotics, and all (100%) were resistant to CIP and NAL. However, TET resistance was observed in 93.9% and 83.3% of and isolates, respectively. Four (8.2%) isolates were multidrug-resistant (MDR), while none of the isolates were MDR. Two of the four MDR isolates were resistant to CIP, NAL, TET, and ERY, whereas the other two isolates were resistant to CIP, NAL, TET, and GEN. The values of the Minimum Inhibitory Concentration (MIC) were as follows: CIP, 64-256 μg/ml; NAL, 128-512 μg/ml; TET, 2-1024 μg/ml; SIT, 0.25-1 μg/ml; ERY, 1-32 μg/ml; and GEN, 1-256 μg/ml. , , , , , , , , and were discovered in more than 50% of isolates, although , , , and were found in <50%. , , , and were discovered in more than 50% of the samples, whereas , , , , and were found in less than half. For , the percentages for , , , and were all zero. The results of this study show isolates obtained from poultry have higher resistance to quinolones and TET, pathogenicity potential, and varied genotypes.
某菌属是全球范围内引起微生物性肠炎最常见的病因之一。本研究的目的是调查从伊朗鸡肉样本中分离出的嗜热某菌属的抗菌药物耐药性（AMR）模式、毒力基因和基因变异情况。共采集了255份肉类标本并送至实验室。采用培养方法鉴定菌属，通过PCR和测序对菌株进行确认。使用肉汤微量稀释法对六种抗菌药物[环丙沙星（CIP）、萘啶酸（NAL）、司帕沙星（SIT）、红霉素（ERY）、四环素（TET）和庆大霉素（GEN）]进行抗菌药物敏感性评估。通过PCR检测AMR和毒力基因。在255份肉类样本中，某菌属的检出率为64株（25.09%），其中某菌A和某菌B分别占41株（64.06%）和14株（21.87%）。其他某菌属分离株占总数的14.06%（9份样本）。使用六种抗生素对所有某菌属分离株进行了药敏试验，所有菌株（100%）对CIP和NAL耐药。然而，某菌A和某菌B分离株中分别有93.9%和83.3%对TET耐药。4株（8.2%）某菌属分离株为多重耐药（MDR），而某菌B分离株均无MDR。4株MDR分离株中有2株对CIP、NAL、TET和ERY耐药，而另外2株分离株对CIP、NAL、TET和GEN耐药。最低抑菌浓度（MIC）值如下：CIP为64 - 256μg/ml；NAL为128 - 512μg/ml；TET为2 - 1024μg/ml；SIT为0.25 - 1μg/ml；ERY为1 - 32μg/ml；GEN为1 - 256μg/ml。在超过50%的某菌属分离株中发现了基因A、基因B、基因C、基因D、基因E、基因F、基因G、基因H和基因I，尽管基因J、基因K、基因L和基因M的发现率低于50%。在超过50%的某菌B样本中发现了基因N、基因O、基因P和基因Q，而基因R、基因S、基因T、基因U和基因V的发现率不到一半。对于某菌C，基因W、基因X、基因Y和基因Z的发现率均为零。本研究结果表明，从家禽中分离出的某菌属分离株对喹诺酮类药物和TET具有较高耐药性、致病潜力且基因型多样。
