Yao Manman, Ren Xujiao, Yin Mengge, Chen Huanchun, Li Xiangmin, Qian Ping
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Nanomedicine (Lond). 2023 Jan;18(1):5-18. doi: 10.2217/nnm-2022-0298. Epub 2023 Feb 15.
To develop a vaccine candidate for Japanese encephalitis virus (JEV), for which an effective and safe vaccine is urgently needed. A vaccine candidate based on domain III of the JEV envelope protein and lumazine synthase (EDIII-LS) was prepared by coupling multivalent ED III to a self-assembling nanoparticle of LS through genetic fusion and self-assembly. High enrichment of ED III was achieved based on the self-assembly of an EDIII-LS polymer. EDIII-LS strongly promoted dendritic cells' internalization and presentation compared with ED III monomer. The cellular and humoral immune responses provoked by EDIII-LS were remarkably higher than those caused by ED III in mice, and conferred complete protection against JEV challenge. The study of ED III-based nanoparticles suggests an effective approach against JEV.
为开发一种日本脑炎病毒（JEV）候选疫苗，目前迫切需要一种有效且安全的疫苗。通过基因融合和自组装将多价的包膜蛋白结构域III（EDIII）与自组装的核黄素合酶纳米颗粒（LS）偶联，制备了一种基于JEV包膜蛋白结构域III和核黄素合酶的候选疫苗（EDIII-LS）。基于EDIII-LS聚合物的自组装实现了EDIII的高度富集。与EDIII单体相比，EDIII-LS强烈促进树突状细胞的内化和呈递。EDIII-LS在小鼠中引发的细胞免疫和体液免疫反应明显高于EDIII引起的反应，并对JEV攻击提供了完全保护。基于EDIII的纳米颗粒研究提示了一种对抗JEV的有效方法。
