Department of Gastroenterology, Wuhan Hospital of Traditional Chinese and Western Medicine, Wuhan, Hubei, China.
Turk J Gastroenterol. 2023 May;34(5):525-532. doi: 10.5152/tjg.2023.22251.
Chronic atrophic gastritis is a premalignant lesion with a high risk of developing into gastric cancer. Rhein is a key active ingredient of several traditional Chinese medicines with multiple pharmacological effects. Nevertheless, the role of rhein in chronic atrophic gastritis is unclear.
Helicobacter pylori infection was used to establish chronic atrophic gastritis in a mouse model. Murine gastric mucosa treated with saline or rhein was used in experiments. Hematoxylin and eosin staining and Alcian blue-periodic acid-Schiff staining were utilized for histopathological observation of murine gastric mucosa. The levels of proinflammatory factors were detected by enzyme-linked immunosorbent assay, and oxidative stress-associated markers were detected by commercially available assay kits. Western blotting was used for measuring the levels of nuclear factor, erythroid 2-like bZIP transcription factor 2, and mitogen-activated protein kinase signaling-related proteins.
Rhein mitigated the gastric mucosal injury and suppressed inflammation and oxidative stress in H. pylori-infected chronic atrophic gastritis mouse models. Rhein inactivated mitogen-activated protein kinase and activated erythroid 2-like bZIP transcription factor 2 signaling in gastric mucosa of mice with chronic atrophic gastritis.
Rhein exhibits anti-inflammatory and antioxidant effects in chronic atrophic gastritis via erythroid 2-like bZIP transcription factor 2 and mitogen-activated protein kinase signaling.
慢性萎缩性胃炎是一种具有较高胃癌发展风险的癌前病变。大黄酸是几种中药的关键活性成分，具有多种药理作用。然而，大黄酸在慢性萎缩性胃炎中的作用尚不清楚。
采用幽门螺杆菌感染建立小鼠慢性萎缩性胃炎模型。用生理盐水或大黄酸处理小鼠胃黏膜进行实验。采用苏木精-伊红染色和阿尔辛蓝-过碘酸希夫染色观察小鼠胃黏膜的组织病理学变化。酶联免疫吸附试验检测促炎因子水平，商业试剂盒检测氧化应激相关标志物。采用 Western blot 法检测核因子、红细胞 2 样 bZIP 转录因子 2 和丝裂原活化蛋白激酶信号相关蛋白的水平。
大黄酸减轻了 H. pylori 感染的慢性萎缩性胃炎小鼠模型的胃黏膜损伤，并抑制了炎症和氧化应激。大黄酸在慢性萎缩性胃炎小鼠胃黏膜中使丝裂原活化蛋白激酶失活，激活了红细胞 2 样 bZIP 转录因子 2 信号。
大黄酸通过红细胞 2 样 bZIP 转录因子 2 和丝裂原活化蛋白激酶信号在慢性萎缩性胃炎中发挥抗炎和抗氧化作用。
