School of Public Health Lanzhou University, Lanzhou 730000, P. R. China.
Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, P. R. China.
Nanoscale. 2023 Mar 9;15(10):5023-5035. doi: 10.1039/d2nr06342e.
Effective and real-time detection of lactate (LA) content in human sweat has attracted considerable attention from researchers. In this work, a novel electrochemical paper-based analysis device (ePAD) was developed for the non-invasive detection of LA in sweat. The electrocatalytic properties of AuNP/Cu-TCPP(Fe) hybrid nanosheets, which were prepared by an optimised synthetic method, were studied by CV and EIS electrochemical methods for the first time and the working electrode can be fabricated using a drip coating method. The lactate sensor was optimised and validated for usability, adoptability and interpretability. To the best of our knowledge, this was the fastest, lowest detection line and widest linear range method reported to date for the detection of lactate. It achieved the detection limit of 0.91 pM and a linear range from 0.013 nM to 100 mM. The dual catalytic effects of the hybrid NSs shortened the detection time by nearly two times and enhanced the sensitivity approximately two times, an accuracy unmatched until now. Furthermore, this sensor was employed for LA analysis and validated by high performance liquid chromatography (HPLC). The ePAD shows superior biocompatibility, accuracy, and high sensitivity and can be easily manufactured. Hence, it is applicable for the long-term monitoring of sweat LA concentrations in point-of-care testing, athletic testing of athletes and military personnel and other subjects in different extreme environments.
有效且实时地检测人体汗液中的乳酸（LA）含量引起了研究人员的极大关注。在这项工作中，开发了一种新型电化学纸基分析装置（ePAD），用于非侵入式检测汗液中的 LA。通过 CV 和 EIS 电化学方法首次研究了通过优化合成方法制备的 AuNP/Cu-TCPP(Fe) 混合纳米片的电催化性能，并且可以使用滴涂法来制造工作电极。对乳酸传感器进行了优化和验证，以确保其可用性、可接受性和可解释性。据我们所知，这是迄今为止报道的检测乳酸的最快、检测线最低和线性范围最宽的方法。它实现了 0.91 pM 的检测限和 0.013 nM 至 100 mM 的线性范围。混合 NSs 的双重催化作用将检测时间缩短了近两倍，灵敏度提高了大约两倍，这是迄今为止无与伦比的准确性。此外，该传感器用于 LA 分析并通过高效液相色谱（HPLC）进行了验证。ePAD 具有出色的生物相容性、准确性和高灵敏度，并且易于制造。因此，它适用于在即时护理测试、运动员和军人及其他处于不同极端环境下的人员的汗液 LA 浓度的长期监测。
