Wang Zhengyun, Dong Shuang, Gui Mengxi, Asif Muhammad, Wang Wei, Wang Feng, Liu Hongfang
Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Anal Biochem. 2018 Feb 15;543:82-89. doi: 10.1016/j.ab.2017.12.010. Epub 2017 Dec 9.
Flexible sweat biosensors are of considerable current interest for the development of wearable smart miniature devices. In this work, we report a novel type of flexible and electrochemical sweat platform fabricated by depositing Cu submicron buds on freestanding graphene paper (GP) carrying MoS nanocrystals monolayer for bio-functional detection of glucose and lactate. Quantitative analysis of glucose and lactate was carried out by using amperometric i-t method. Linear ranges were obtained between 5 and 1775 μM for glucose and 0.01-18.4 mM for lactate, and their corresponding limits of detection were 500 nM and 0.1 μM, respectively. The platform demonstrates fast response, good selectivity, superb reproducibility and outstanding flexibility, which enable its use for monitoring glucose and lactate in human perspiration. The strategy of structurally integrating 3D transition metal, 0D transition metal sulfide and 2D graphene will provide new insight into the design of flexible electrodes for sweat glucose and lactate monitoring and a wider range of applications in biosensing, bioelectronics, and lab-on-a-chip devices.
柔性汗液生物传感器对于可穿戴智能微型设备的发展具有相当大的当前研究兴趣。在这项工作中，我们报道了一种新型的柔性电化学汗液平台，该平台通过在负载有单层MoS纳米晶体的独立石墨烯纸（GP）上沉积亚微米级铜芽来制备，用于葡萄糖和乳酸的生物功能检测。采用安培i-t法对葡萄糖和乳酸进行定量分析。葡萄糖的线性范围为5至1775μM，乳酸的线性范围为0.01至18.4 mM，它们相应的检测限分别为500 nM和0.1μM。该平台表现出快速响应、良好的选择性、出色的重现性和卓越的柔韧性，使其能够用于监测人体汗液中的葡萄糖和乳酸。将三维过渡金属、零维过渡金属硫化物和二维石墨烯进行结构整合的策略，将为用于汗液葡萄糖和乳酸监测的柔性电极设计提供新的思路，并在生物传感、生物电子学和芯片实验室设备中有更广泛的应用。
