School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005711.
A novel yeast species is described based on three strains isolated from rotting wood samples from Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Strain NYNU 1811121 was isolated in Menglun, Mengla, while strains NYNU 18982 and NYNU 181096 were recovered in Mengyang, Jinghong. Analysis of the sequences of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) revealed that the novel strains were closely related to the type strain of [] , but with 6.9 % nucleotide substitutions in the D1/D2 domain and 8.2 % substitutions in the ITS region. The three novel strains can also be distinguished from in terms of the ability to assimilate trehalose and d-gluconate and to grow at 35 °C, as well as the inability to ferment glucose. Based on molecular analyses and phenotypic characteristics, the name f.a., sp. nov. is proposed with the holotype CBS 16011 (MycoBank 845375).
描述了一个新的酵母物种,该物种基于从中国云南省西双版纳热带雨林腐烂木材样本中分离的三个菌株。菌株 NYNU 1811121 是在勐仑分离得到的,而菌株 NYNU 18982 和 NYNU 181096 是在勐养分离得到的。对大亚基 rRNA 基因的 D1/D2 结构域和内部转录间隔区(ITS)区域(ITS1-5.8S-ITS2)的序列分析表明,新型菌株与 []的模式菌株密切相关,但在 D1/D2 结构域中有 6.9%的核苷酸取代,在 ITS 区域中有 8.2%的取代。这三个新菌株还可以通过同化海藻糖和 d-葡萄糖酸的能力以及在 35°C 下生长的能力,以及不能发酵葡萄糖来与 []区分开来。基于分子分析和表型特征,提议使用模式菌株 CBS 16011(MycoBank 845375)来命名为 []f.a.,sp. nov.。