Chu Shuai-Bei, Hu Wen-Ting, Hui Feng-Li
School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005629.
Two strains of a novel ascomycetous yeast species were isolated from rotting wood samples collected in Jiuxi Mountain Forest Park in Yunnan Province, southwest China. Both strains formed one or two spherical ascospores in persistent asci. Phylogenetic analysis of the concatenated sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) and the D1/D2 domain of the large subunit rRNA gene revealed that the novel strains represented a phylogenetically distinct species belonging to the genus . This novel species differed from the type strains of the closest known species, and , by 0.9 and 1.2 % nucleotide substitutions in the D1/D2 domain and 5.3 and 6 % nucleotide substitutions in the ITS region, respectively. The novel species can also be distinguished from and in terms of the ability to assimilate ribitol, succinate and citrate, and its ability to grow at 37 °C. The species name of sp. nov. is proposed with holotype CBS 16004 (Mycobank MB 844535).
从中国西南部云南省九溪山森林公园采集的腐烂木材样本中分离出两株新型子囊菌酵母菌种。两株菌在持久的子囊中形成一个或两个球形子囊孢子。对内部转录间隔区（ITS）区域（ITS1-5.8S-ITS2）和大亚基rRNA基因的D1/D2结构域的串联序列进行系统发育分析表明，这些新型菌株代表了一个在系统发育上属于该属的不同物种。这个新物种与最接近的已知物种的模式菌株相比，在D1/D2结构域中核苷酸替换分别为0.9%和1.2%，在ITS区域中核苷酸替换分别为5.3%和6%。这个新物种在同化核糖醇、琥珀酸盐和柠檬酸盐的能力以及在37℃下生长的能力方面也可以与其他物种区分开来。提出了该新物种的名称为sp. nov.，模式菌株为CBS 16004（Mycobank MB 844535）。
