Zheng Jun, Lu Yun-Feng, Liu Xiao-Jing, Hui Feng-Li
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5051-5055. doi: 10.1099/ijsem.0.002411. Epub 2017 Oct 12.
Three strains representing a novel species of yeast were isolated from samples of rotting wood collected from Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that the novel species is a member of the genus Cyberlindnera, although the formation of ascospores was not observed. The novel species was related most closely to the type strain of the species Candida pattaniensis, but they had a 0.5 % sequence divergence (3 substitutions, 0 gap) in the D1/D2 domain and a 5.4 % sequence divergence (21 substitutions, 10 gaps) in the ITS region. The novel species could also be differentiated from the closely related species by some biochemical and physiological characteristics. The species name Cyberlindnera xishuangbannaensis f.a., sp. nov. is proposed to accommodate these strains, with NYNU 16752 (=CICC 33163=CBS 14692) designated as the type strain. The MycoBank number is MB 822199.
从中国云南省西双版纳热带雨林采集的腐烂木材样本中分离出了代表一种新型酵母的三株菌株。基于内部转录间隔区(ITS)区域和大亚基(LSU)rRNA基因的D1/D2结构域的串联序列进行的系统发育分析表明,该新物种是Cyberlindnera属的成员,尽管未观察到子囊孢子的形成。该新物种与帕塔尼假丝酵母(Candida pattaniensis)的模式菌株关系最为密切,但它们在D1/D2结构域中的序列差异为0.5%(3个替换,0个空位),在ITS区域中的序列差异为5.4%(21个替换,10个空位)。该新物种也可通过一些生化和生理特征与密切相关的物种区分开来。提出将该物种命名为西双版纳Cyberlindnera(Cyberlindnera xishuangbannaensis f.a., sp. nov.)以容纳这些菌株,指定NYNU 16752(=CICC 33163=CBS 14692)为模式菌株。其MycoBank编号为MB 822199。