Nanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Aragon Institute of Nanoscience, University of Zaragoza, 50009, Saragossa, Spain.
Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, 50009, Saragossa, Spain.
Anal Bioanal Chem. 2023 Apr;415(9):1777-1786. doi: 10.1007/s00216-023-04583-0. Epub 2023 Feb 15.
Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) have been immobilized on cellulose to obtain disposable biosensors for the determination of histamine. During the enzymatic reaction, AR is oxidized and a pink spot is obtained. Using a smartphone and measuring the G (green) color coordinate, histamine can be determined in the presence of other biogenic amines (putrescine and cadaverine) in concentrations ranging from 2·10 M to 5·10 M with a 7.5·10 M limit of detection (LoD). Despite tyramine interference, experimental conditions are provided which allow rapid and simple histamine and simultaneous histamine/tyramine (semi)quantitative determination in mixtures. Finally, tyramine and histamine were determined in a tuna extract with good results (compared to the reference HPLC-MS method). The methodology can also be applied in solution allowing histamine (and simultaneous histamine/tyramine) determination with a lower LoD (1.8·10 M) and a similar selectivity.
酪氨酸氧化酶（TAO）、过氧化物酶（HRP）和 Amplex Red（AR）已被固定在纤维素上，以获得用于测定组氨酸的一次性生物传感器。在酶反应过程中，AR 被氧化，得到一个粉红色斑点。使用智能手机并测量 G（绿色）颜色坐标，可以在存在其他生物胺（腐胺和尸胺）的情况下，在 2·10~5·10 M 的浓度范围内检测组胺，检测限（LoD）为 7.5·10 M。尽管存在酪胺干扰，但提供了实验条件，可以快速简单地进行组胺和同时的组胺/酪胺（半）定量测定混合物。最后，在金枪鱼提取物中测定了酪胺和组胺，结果良好（与参考 HPLC-MS 方法相比）。该方法也可用于溶液中，允许以较低的 LoD（1.8·10 M）和类似的选择性测定组胺（和同时的组胺/酪胺）。
