Steffan A M, Gendrault J L, Kirn A
INSERM U 74, Faculté de Médecine de Strasbourg, France.
Hepatology. 1987 Nov-Dec;7(6):1230-8. doi: 10.1002/hep.1840070610.
Endothelial cells of the hepatic sinusoid isolated from mice livers and maintained in culture display typical fenestrae grouped in sieve plates. Treatment with cytochalasin B led to no significant change in the mean diameter of the fenestrae but to an increase in their number and in the porosity of the cells (percentage of the cellular surface opened by the fenestrae) which attained up to 300% of that of the controls. Scanning electron microscopic observations of Triton-extracted cells revealed that these modifications were related to an alteration of the cytoskeleton. The effect of cytochalasin B could be reversed; 3 hr after removal of the drug, the cells recovered their original aspect with sieve plates scattered over their surface. These observations demonstrate that endothelial fenestrae are inducible structures and that the cytoskeleton seems to be involved in their formation.
从小鼠肝脏分离并在培养中维持的肝血窦内皮细胞呈现出典型的、成筛板状排列的窗孔。用细胞松弛素B处理后，窗孔的平均直径没有显著变化，但窗孔数量及其孔隙率（窗孔所开放的细胞表面积百分比）增加，最高达到对照组的300%。对经曲拉通处理的细胞进行扫描电子显微镜观察发现，这些改变与细胞骨架的改变有关。细胞松弛素B的作用可以逆转；去除药物3小时后，细胞恢复其原始外观，筛板分散在其表面。这些观察结果表明，内皮窗孔是可诱导的结构，并且细胞骨架似乎参与了它们的形成。
