Anjum Nazifa, Hossain Md Saddam, Rahman Md Atiar, Rafi Md Khalid Juhani, Al Noman Abdullah, Afroze Mirola, Saha Srabonti, Alelwani Walla, Tangpong Jitbanjong
Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, 4331, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong, 4331, Bangladesh; School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
J Ethnopharmacol. 2023 May 23;308:116189. doi: 10.1016/j.jep.2023.116189. Epub 2023 Feb 13.
Diarrhea is one of the leading causes of preventable death in developing countries, mainly caused by bacterial infections and traditional therapies are very common in diarrheal incidences. Meda Pata (Litsea glutionsa) has a long history of use as traditional medicine for diarrhea, dysentery, and spasm in Bangladesh, India, and some other Asian countries.
This research reports the antidiarrheal effects of Meda Pata (Litsea glutinosa leaf extract, LGLEx) in animal models. The work has been supported by in silico molecular docking study to verify the effects.
The antidiarrheal effect of LGLEx was investigated in castor oil-induced diarrhea, magnesium sulfate-induced diarrhea, and gastrointestinal motility test models. Antidiarrheal effects were supported by a molecular docking study through an interaction between LGLEx's GC-MS analyzed imidazole-containing compounds and muscarinic acetylcholine receptor (PDB: 4U14) and 5-HT3 receptor (PDB: 5AIN).
LGLEx potentially reduced the diarrheal incidences in in vivo assays reducing gastrointestinal motility. The maximum diarrheal inhibition was obtained in the castor oil-induced model (62.63%) and and BaSO-induced model (73.14%), which were statistically significant (P < 0.05) when compared to the reference drug loperamide. In the castor-oil and BaSO4-induced models, peristaltic movement was reduced by 25.96% and 32.17%, respectively. Biochemical markers particularly IgE, C-reactive proteins, and serum electrolytes were significantly (P < 0.0) restored in treated groups. A Molecular docking analysis revealed that two compounds (1-Ethyl-2-hydroxymethylimidazole and 1,6-Anhydro-beta-D-glucofuranose demonstrated the highest binding affinity with target receptors muscarinic acetylcholine receptor (PDB: 4U14) and 5-HT3 receptor (PDB: 5AIN) confirming their drug likeliness. The findings indicate a high potential antidiarrheal impact that warrants further investigation for its therapeutic application.
腹泻是发展中国家可预防死亡的主要原因之一，主要由细菌感染引起，传统疗法在腹泻发病率中非常常见。在孟加拉国、印度和其他一些亚洲国家，梅达·帕塔（Litsea glutionsa）作为治疗腹泻、痢疾和痉挛的传统药物有着悠久的使用历史。
本研究报告了梅达·帕塔（Litsea glutinosa叶提取物，LGLEx）在动物模型中的止泻作用。这项工作得到了计算机分子对接研究的支持，以验证其效果。
在蓖麻油诱导的腹泻、硫酸镁诱导的腹泻和胃肠动力测试模型中研究了LGLEx的止泻作用。通过LGLEx的气相色谱-质谱分析含咪唑化合物与毒蕈碱型乙酰胆碱受体（PDB：4U14）和5-羟色胺3型受体（PDB：5AIN）之间的相互作用，分子对接研究支持了止泻作用。
LGLEx在体内试验中潜在地降低了腹泻发病率，降低了胃肠动力。在蓖麻油诱导的模型（62.63%）和硫酸钡诱导的模型（73.14%）中获得了最大的腹泻抑制率，与参比药物洛哌丁胺相比，具有统计学意义（P<0.05）。在蓖麻油和硫酸钡诱导的模型中，蠕动分别减少了25.96%和32.17%。治疗组的生化标志物特别是免疫球蛋白E、C反应蛋白和血清电解质显著（P<0.0）恢复。分子对接分析表明，两种化合物（1-乙基-2-羟甲基咪唑和1,6-脱水-β-D-葡萄糖呋喃糖）与靶受体毒蕈碱型乙酰胆碱受体（PDB：4U14）和5-羟色胺3型受体（PDB：5AIN）表现出最高的结合亲和力，证实了它们的药物可能性。这些发现表明其具有很高的潜在止泻作用，值得进一步研究其治疗应用。
