Department of Pharmacology & Therapeutics, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B. 4400 Ogbomoso, Nigeria.
J Ethnopharmacol. 2011 Sep 1;137(1):148-53. doi: 10.1016/j.jep.2011.04.068. Epub 2011 May 6.
Pyrenacantha staudtii Engl. (Icacinaceae) is a plant which is traditionally used for the treatment of blemnorrhea, hernia, insomnia, intestinal pain and diarrhea in Nigeria. Therefore the core aim of the present study is to evaluate antidiarrheal activity of Pyrenacantha staudtii aqueous extract (PSE).
The antidiarrheal activity was evaluated using castor oil-induced diarrhea method. The effects of Pyrenacantha staudtii aqueous extract on gastrointestinal motility, intestinal transit and enteropooling were also examined in rodents. The acute toxicity effect of the aqueous extract of Pyrenacantha staudtii was also investigated.
Pyrenacantha staudtii aqueous extract (PSE, 100-400mg/kg, p.o.) produced dose-dependent and significant (P<0.05-0.01) protection of rats and mice against castor oil-induced diarrhea, inhibited intestinal transit, and delayed gastric emptying. PSE, produced dose-dependent and significant (P<0.05-0.01) antimotility effect, caused dose-related inhibition of castor-oil-induced enteropooling in animals, comparable to atropine (1mg/kg, p.o.). Like loperamide (10mg/kg, p.o.), PSE, dose-dependently and significantly (P<0.05-0.01) delayed the onset of castor-oil induced diarrhea, decreased the frequency of defecation, and reduced the severity of diarrhea in the rodents. Compared with control animals, PSE, dose-dependently and significantly (P<0.05-0.01) decreased the volume of castor oil-induced intestinal fluid secretion, and reduced the number, weight and wetness of fecal droppings.
The findings of this study indicate that PSE possesses antidiarrheal property in rats and mice. These findings confirm the ethnomedicinal use of Pyrenacantha staudtii leaf as a valuable natural remedy for the treatment, management and/or control of diarrhea.
Pyrenacantha staudtii Engl.（铁青树科）是一种植物，在尼日利亚传统上用于治疗 blemnorrhea、疝气、失眠、腹痛和腹泻。因此，本研究的核心目标是评估 Pyrenacantha staudtii 水提物（PSE）的抗腹泻活性。
采用蓖麻油诱导腹泻法评价抗腹泻活性。还在啮齿动物中检查了 Pyrenacantha staudtii 水提取物对胃肠道动力、肠传输和肠液积聚的影响。还研究了 Pyrenacantha staudtii 水提取物的急性毒性作用。
Pyrenacantha staudtii 水提取物（PSE，100-400mg/kg，po）对大鼠和小鼠产生了剂量依赖性和显著的（P<0.05-0.01）蓖麻油诱导腹泻保护作用，抑制了肠传输，并延迟了胃排空。PSE 产生了剂量依赖性和显著的（P<0.05-0.01）抗运动作用，导致动物中蓖麻油诱导的肠液积聚呈剂量相关抑制，与阿托品（1mg/kg，po）相当。与洛哌丁胺（10mg/kg，po）一样，PSE 剂量依赖性和显著（P<0.05-0.01）延迟了蓖麻油诱导的腹泻发作，减少了排便频率，并降低了啮齿动物的腹泻严重程度。与对照动物相比，PSE 剂量依赖性和显著（P<0.05-0.01）降低了蓖麻油诱导的肠道液体分泌量，减少了粪便中粪便的数量、重量和湿度。
本研究的结果表明，PSE 对大鼠和小鼠具有抗腹泻作用。这些发现证实了 Pyrenacantha staudtii 叶作为治疗、管理和/或控制腹泻的有价值天然药物的民族医学用途。
