Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), P.O. Box 31485498, Karaj, Iran.
Department of Landscape Engineering, Faculty of Agriculture, Malayer University, Malayer, 6571995863, Iran.
Sci Rep. 2023 Feb 15;13(1):2713. doi: 10.1038/s41598-023-29896-z.
Ionizing radiations are a helpful technique and have improved financial potential in developing new and unique commercially important fruit tree varieties. The 'Yaghouti' grape cuttings were treated with 0 (control), 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy gamma ray (γ) doses. The objectives of this study were to (1) investigate the effects of γ radiation on the survival rate, leaves, shoots, and root morphometric traits after γ irradiation; (2) Measurement of the 25, 50, and 75% lethal dose and 25, 50, and 75% growth reduction dose based on leaves, shoots, and root morphometric characteristics to estimate grape radiosensitivity; (3) Ultimately, determining the optimum dose of γ irradiation based biological responses (LD and GR) in Yaghouti grape. The findings demonstrate that the lethal dose of the 'Yaghouti' was 18 Gy for LD, 30 Gy for LD, and 48 Gy for LD, respectively. Furthermore, our findings showed that increasing the γ dose had a harmed ngative effect on vine growth, as evidenced by a decrease in plant height, root number, root volume, leaf area, aerial biomass, root biomass, and internode number of the 'Yaghouti' grape plants. Our results showed that between the aerial parts of vines, the leaf area and aerial biomass had higher radiosensitivity than plant height and other aerial parts of the plants based on data from GR, GR, and GR. Moreover, GR studies of root characteristics revealed that root number and biomass root had higher radiation sensitivity than root volume. According to biological responses (LD and GR) in the 'Yaghouti' grape, 30 Gy of γ radiation is the optimum dose for preliminary mutagenesis investigations.
电离辐射是一种有用的技术，它提高了开发新的和独特的具有商业重要性的果树品种的经济潜力。“Yaghouti”葡萄插条用 0（对照）、10、20、30、40、50、60、70、80、90 和 100Gy 伽马射线（γ）剂量处理。本研究的目的是：（1）研究γ射线照射对γ射线照射后成活率、叶片、枝条和根系形态特征的影响；（2）根据叶片、枝条和根系形态特征，测量 25%、50%和 75%致死剂量和 25%、50%和 75%生长抑制剂量，估计葡萄的辐射敏感性；（3）最终根据 Yaghouti 葡萄的生物响应（LD 和 GR）确定γ辐照的最佳剂量。研究结果表明，‘Yaghouti’的致死剂量为 18Gy 为 LD，30Gy 为 LD，48Gy 为 LD。此外，我们的研究结果表明，随着γ剂量的增加，葡萄生长受到了损害，表现为株高、根数量、根体积、叶面积、地上生物量、根生物量和节间数的减少。我们的研究结果表明，在葡萄植株的地上部分中，叶片面积和地上生物量比株高和其他地上部分具有更高的辐射敏感性，这是基于 GR、GR 和 GR 的数据。此外，根特征的 GR 研究表明，根数量和根生物量比根体积具有更高的辐射敏感性。根据‘Yaghouti’葡萄的生物响应（LD 和 GR），30Gy 的γ辐射是初步诱变研究的最佳剂量。
