Department of Vegetable Science, Faculty of Horticulture, Nadia, India.
Department of Agricultural Statistics, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, India.
Int J Radiat Biol. 2023;99(3):534-550. doi: 10.1080/09553002.2022.2107723. Epub 2022 Aug 9.
Three experiments were conducted to assess the effect of different doses of gamma radiation on various seedling traits; determine the optimum doses of gamma radiation for different faba bean genotypes; find out the variation in optimum doses with respect to the different times of sowings after irradiation and methods of irradiation.
Five faba bean genotypes ., L-2013-060, L-2013-092, Anandnagar Local, Gazipur Local and Bangla Gangachar were used in these experiments. In Experiment I, seeds of five experimental genotypes were exposed to different doses (100 Gy 200 Gy, 300 Gy, 400 Gy, 500 Gy, 600 Gy, 700 Gy and 800 Gy) of gamma radiation and were sown immediately after irradiation. In Experiment II, seeds of Bangla Gangachar and L-2013-060 were exposed to varying doses (100-800 Gy) of gamma radiation and were sown at seven sowings starting from 0 h to 24 h at 4-h intervals after irradiation. In Experiment III, L-2013-092 genotypes was exposed to different doses (100 -800 Gy) of gamma radiation with two different methods of irradiation.
In Experiment I, the lethal dose 50 (LD) values have arrived at 140 Gy, 669 Gy, 575 Gy, 386 Gy and 158 Gy for L-2013-060, L-2013-092, Anandnagar Local, Gazipur Local and Bangla Gangachar, respectively. The growth reduction 50 (GR) doses for different seedling traits ranged from 130 Gy to 320 Gy for L-2013-060, 250 Gy to 480 Gy for L-2013-092, 130 Gy to 370 Gy for Anandnagar Local, 200 Gy to 350 Gy for Gazipur Local and 250 Gy to 400 Gy for Bangla Gangachar. In Experiment II, the values for LD of the genotypes Bangla Gangachar and L-2013-060 were significantly singular for different time intervals of sowing. The values of GR for most of the seedling traits were found to increase with the delay in sowing after irradiation from 4 to 24 h when compared with the immediately sown seed lots. In Experiment III, LD for L-2013-092 was 337 Gy with Method 1 and 669 Gy with Method 2. In Method 1, most of the growth parameters attained GR doses lower than Method 2. The first method was found to increase the radiosensitivity of L-2013-092.
Every experimental genotype used in these three experiments showed dose-dependent retardation of different seedling traits. These optimized doses may be employed to establish mutant populations for exploiting the novel traits of faba bean. The time of sowing after irradiation and method of irradiation was found to be essential for confirming optimum doses.
进行了三项实验，以评估不同剂量的伽马辐射对各种幼苗特征的影响；确定不同 faba 豆基因型的伽马辐射最佳剂量；找出不同辐照后播种时间和辐照方法下最佳剂量的变化。
使用了五种 faba 豆基因型。L-2013-060、L-2013-092、安纳德纳加尔本地、加济布尔本地和 Bangla Gangachar。在实验 I 中，将五种实验基因型的种子暴露于不同剂量（100Gy、200Gy、300Gy、400Gy、500Gy、600Gy、700Gy 和 800Gy）的伽马辐射下，并在辐照后立即播种。在实验 II 中，将 Bangla Gangachar 和 L-2013-060 的种子暴露于不同剂量（100-800Gy）的伽马辐射下，并在辐照后 0 至 24 小时以 4 小时的间隔进行 7 次播种。在实验 III 中，将 L-2013-092 基因型暴露于不同剂量（100-800Gy）的伽马辐射下，并采用两种不同的辐照方法。
在实验 I 中，L-2013-060、L-2013-092、安纳德纳加尔本地、加济布尔本地和 Bangla Gangachar 的致死剂量 50（LD）值分别为 140Gy、669Gy、575Gy、386Gy 和 158Gy。不同幼苗特征的生长减少 50（GR）剂量范围为 L-2013-060 的 130Gy 至 320Gy、L-2013-092 的 250Gy 至 480Gy、安纳德纳加尔本地的 130Gy 至 370Gy、加济布尔本地的 200Gy 至 350Gy 和 Bangla Gangachar 的 250Gy 至 400Gy。在实验 II 中，基因型 Bangla Gangachar 和 L-2013-060 的 LD 值在不同播种时间间隔下显著不同。与立即播种的种子相比，大多数幼苗特征的 GR 值随着辐照后播种时间从 4 小时延迟到 24 小时而增加。在实验 III 中，L-2013-092 的 LD 值在方法 1 下为 337Gy，在方法 2 下为 669Gy。在方法 1 下，大多数生长参数的 GR 剂量低于方法 2。发现第一种方法增加了 L-2013-092 的放射敏感性。
这三项实验中使用的每个实验基因型都表现出不同幼苗特征的剂量依赖性延迟。这些最佳剂量可用于建立突变体群体，以开发 faba 豆的新特性。辐照后播种时间和辐照方法被发现是确定最佳剂量的关键。
