Airoldi L, Bonfanti M, Magagnotti C, Fanelli R
Laboratory of Environmental Pharmacology and Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
IARC Sci Publ. 1987(84):159-61.
The capacity of the isolated rat urinary bladder to metabolize chemical carcinogens was studied. Under our experimental conditions, the bladder carcinogen N-nitrosobutyl-(4-hydroxybutyl)amine (NBHBA) was oxidized to N-nitrosobutyl(3-carboxypropyl)amine (NBCPA). A time-dependent increase in the amount of NBCPA formed and a simultaneous disappearance of NBHBA indicated that the bladder can metabolize NBHBA to the metabolite considered to be responsible for tumour induction in the urinary bladder of laboratory animals. After 15, 30, 60 and 120 min, the percentages of NBCPA formed were 10%, 21%, 35% and 61%, respectively, and 59%, 49%, 36% and 25% of NBHBA remained unchanged. When N-nitrosodi-n-butylamine (NDBA) was introduced into the isolated urinary bladder and incubated for 120 min, its oxidized metabolites NBHBA and NBCPA were formed, in amounts of 0.13% and 0.06% of the substrate added.
对分离出的大鼠膀胱代谢化学致癌物的能力进行了研究。在我们的实验条件下，膀胱致癌物N-亚硝基丁基-（4-羟基丁基）胺（NBHBA）被氧化为N-亚硝基丁基（3-羧丙基）胺（NBCPA）。形成的NBCPA量随时间增加，同时NBHBA消失，这表明膀胱可将NBHBA代谢为被认为是实验动物膀胱肿瘤诱发原因的代谢物。15、30、60和120分钟后，形成的NBCPA百分比分别为10%、21%、35%和61%，未改变的NBHBA分别为59%、49%、36%和25%。当将N-亚硝基二正丁胺（NDBA）引入分离的膀胱并孵育120分钟时，形成了其氧化代谢物NBHBA和NBCPA，其量分别为添加底物的0.13%和0.06%。
