Departamento de Ingenierías Química y Bioquímica, TecNM/Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., 34080 Durango, Mexico.
Biotechnology Department, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, 09340 Mexico City, Mexico.
Lett Appl Microbiol. 2023 Feb 16;76(2). doi: 10.1093/lambio/ovac061.
Nowadays, isoamyl acetate production is carried out by chemical synthesis with a recent interest in developing biological producing processes, mainly based on microorganisms in submerged fermentation. This work assayed producing isoamyl acetate through solid-state fermentation (SSF), feeding the precursor in the gas phase. Polyurethane foam functioned as the inert support to contain 20 ml of a solution of molasses (10% w/v, pH 5.0). The yeast Pichia fermentans was inoculated at 3 × 107 cells per gram of initial dry weight. The airstream to supply oxygen also served to supply the precursor. Slow supply was obtained using an isoamyl alcohol solution of 5 g l-1 in the bubbling columns and an air stream of 50 ml min-1. For fast supply, fermentations were aerated using 10 g l-1 and 100 ml min-1 for isoamyl alcohol solution and air stream, respectively. It demonstrated the feasibility of isoamyl acetate production in SSF. Moreover, the slow supply of the precursor increased isoamyl acetate production up to 390 mg l-1, which is 12.5 times higher than that obtained without precursor (32 mg l-1). On the other hand, fast supply caused an evident inhibition of the growth and production capacity of the yeast.
如今，乙酸异戊酯的生产是通过化学合成进行的，最近人们对开发基于微生物发酵的生物生产工艺产生了兴趣，主要是在浸没发酵中。本工作通过固态发酵（SSF），在气相中添加前体来生产乙酸异戊酯。聚氨酯泡沫作为惰性载体，含有 20ml 浓度为 10%（w/v）、pH 值为 5.0 的糖蜜溶液。酵母 Pichia fermentans 以每克初始干重 3×107 个细胞的接种量接种。气流不仅为微生物提供氧气，也为其提供前体。通过在鼓泡柱中使用 5g l-1 的乙酸异戊酯溶液和 50ml min-1 的空气流来实现缓慢供应。为了快速供应，发酵分别使用 10g l-1 和 100ml min-1 的乙酸异戊酯溶液和空气流进行通气。这证明了在 SSF 中生产乙酸异戊酯的可行性。此外，缓慢供应前体将乙酸异戊酯的产量提高到 390mg l-1，是不添加前体（32mg l-1）时的 12.5 倍。另一方面，快速供应会明显抑制酵母的生长和生产能力。
