Camargo Aline Frumi, Dalastra Caroline, Ulrich Alessandro, Scapini Thamarys, Bonatto Charline, Klanovicz Natalia, Michelon William, Lerin Lindomar, Júnior Sérgio Luiz Alves, Mossi Altemir José, Tramontin Marco A, Bernardi Oderlei, Paudel Shukra Raj, Fongaro Gislaine, Treichel Helen
Graduate Program in Biotechnology and Biosciences, Federal University of Santa Catarina, Florianópolis, Brazil.
Laboratory of Microbiology and Bioprocesses, Federal University of Fronteira Sul, Erechim, Brazil.
Bioprocess Biosyst Eng. 2023 May;46(5):665-679. doi: 10.1007/s00449-023-02852-x. Epub 2023 Feb 16.
This study evaluated the bioherbicidal potential of wild fungi grown on microalgal biomass from the digestate treatment of biogas production. Four fungal isolates were used and the extracts were evaluated for the activity of different enzymes and characterized by gas chromatography coupled with mass spectrometry. The bioherbicidal activity was assessed by application on Cucumis sativus, and the leaf damage was visually estimated. The microorganisms showed potential as agents producing an enzyme pool. The obtained fungal extracts presented different organic compounds, most acids, and when applied to Cucumis sativus, showed high levels of leaf damage (80-100 ± 3.00%, deviation relative to the observed average damage). Therefore, the microbial strains are potential biological control agents of weeds, which, together with the microalgae biomass, offer the appropriate conditions to obtain an enzyme pool of biotechnological relevance and with favorable characteristics to be explored as bioherbicides, addressing aspects within the environmental sustainability.
本研究评估了在沼气生产消化液处理产生的微藻生物质上生长的野生真菌的生物除草潜力。使用了四种真菌分离物，并对提取物的不同酶活性进行了评估，并通过气相色谱-质谱联用进行了表征。通过施用于黄瓜来评估生物除草活性，并目测估计叶片损伤情况。这些微生物显示出作为产生酶库的试剂的潜力。所获得的真菌提取物呈现出不同的有机化合物，大多数为酸，当施用于黄瓜时，显示出高水平的叶片损伤（80-100±3.00%，相对于观察到的平均损伤的偏差）。因此，这些微生物菌株是杂草的潜在生物防治剂，它们与微藻生物质一起，提供了适当的条件来获得具有生物技术相关性且具有作为生物除草剂进行探索的有利特性的酶库，解决了环境可持续性方面的问题。
