Graduate Program in Biotechnology and Biosciences, Federal University of Santa Catarina, Florianópolis, Brazil.
Laboratory of Microbiology and Bioprocesses, Federal University of Fronteira Sul, Erechim, Brazil.
Bioprocess Biosyst Eng. 2024 May;47(5):651-663. doi: 10.1007/s00449-024-02991-9. Epub 2024 Mar 30.
During scaling of fermentations, choosing a bioreactor is fundamental to ensure the product's quality. This study aims to produce bioherbicides using Trichoderma koningiopsis fermentation, evaluating process parameters in an Airlift bioreactor. As a response, we quantified the production of enzymes involved in the bioherbicide activity (amylase, cellulase, laccase, lipase, and peroxidase). In addition, it evaluated the agronomic efficiency of the fermented extract optimized through tests that promoted soybean growth and nodulation, soybean seed germination, and in vitro phytopathogen control. As a result of optimizing the scaling bioprocess, it was possible to obtain an adequate fermentation condition, which, when applied to soybean seeds, had beneficial effects on their growth. It allowed the production of an enzyme cocktail. These results add a crucial biotechnological potential factor for the success of the optimized formulation in the Airlift bioreactor, in addition to presenting relevant results for the scientific community.
在发酵放大过程中，选择合适的生物反应器是确保产品质量的关键。本研究旨在利用康宁木霉发酵生产生物除草剂，评估气升式生物反应器中的工艺参数。作为响应，我们量化了参与生物除草剂活性的酶的生产（淀粉酶、纤维素酶、漆酶、脂肪酶和过氧化物酶）。此外，还评估了通过促进大豆生长和结瘤、大豆种子发芽以及体外植物病原菌控制的试验优化发酵提取物的农业效率。通过优化放大生物过程，获得了合适的发酵条件，将其应用于大豆种子上，对其生长有有益的影响。它允许生产酶混合物。这些结果为优化配方在气升式生物反应器中的成功应用增加了一个关键的生物技术潜力因素，同时也为科学界提供了相关的结果。
