Garaiova Martina, Hua Qiang, Holic Roman
Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 84005, Slovakia.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
J Agric Food Chem. 2023 Mar 1;71(8):3842-3851. doi: 10.1021/acs.jafc.2c08967. Epub 2023 Feb 16.
Calendic acid (CA) is a conjugated fatty acid with anti-cancer properties that is widely present in seed oil of . Using the co-expression of fatty acid conjugases (CoFADX-1 or CoFADX-2) and fatty acid desaturase (PgFAD2), we metabolically engineered the synthesis of CA in the yeast without the need for linoleic acid (LA) supplementation. The highest CA titer and achieved accumulation were 4.4 mg/L and 3.7 mg/g of DCW in PgFAD2 + CoFADX-2 recombinant strain cultivated at 16 °C for 72 h, respectively. Further analyses revealed the accumulation of CA in free fatty acids (FFA) and downregulation of the gene encoding long-chain fatty acyl-CoA synthetase. The developed recombinant yeast system represents an important tool for the future identification of the essential components of the channeling machinery to produce CA as a high-value conjugated fatty acid at an industrial level.
calendic酸（CA）是一种具有抗癌特性的共轭脂肪酸，广泛存在于……的种子油中。通过脂肪酸共轭酶（CoFADX-1或CoFADX-2）和脂肪酸去饱和酶（PgFAD2）的共表达，我们在酵母中对CA的合成进行了代谢工程改造，无需补充亚油酸（LA）。在16℃培养72小时的PgFAD2 + CoFADX-2重组菌株中，最高CA滴度和积累量分别为4.4 mg/L和3.7 mg/g干细胞重。进一步分析表明，CA在游离脂肪酸（FFA）中积累，并且编码长链脂肪酰辅酶A合成酶的基因表达下调。所开发的重组酵母系统是未来鉴定用于在工业水平生产作为高价值共轭脂肪酸的CA的通道机制必需成分的重要工具。
