Matson Robert S
QuantiScientifics LLC, Orange, CA, USA.
Methods Mol Biol. 2023;2612:141-155. doi: 10.1007/978-1-0716-2903-1_11.
Cytokines are well known to be involved in numerous biological responses with diverse mechanisms of action, including the inflammatory process. The so-called "cytokine storm" has recently been associated with cases of severe COVID-19 infection.Lateral flow microarray (LFM) devices have been constructed for multiplex detection of cytokines. The LFM-cytokine rapid test involves the immobilization of an array of capture anti-cytokine antibodies. Here, we describe the methods to create and use multiplex lateral flow-based immunoassays based upon the enzyme-linked immunosorbent assay (ELISA).
众所周知，细胞因子参与众多具有不同作用机制的生物学反应，包括炎症过程。最近，所谓的“细胞因子风暴”与重症新型冠状病毒肺炎感染病例有关。已构建侧向流动微阵列（LFM）装置用于细胞因子的多重检测。LFM细胞因子快速检测涉及固定一系列捕获抗细胞因子抗体。在此，我们描述基于酶联免疫吸附测定（ELISA）创建和使用多重侧向流动免疫测定的方法。
