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侧向流动检测法同时检测细胞免疫和体液免疫应答。

Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

机构信息

Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.

出版信息

Clin Biochem. 2011 Oct;44(14-15):1241-6. doi: 10.1016/j.clinbiochem.2011.06.983. Epub 2011 Jul 6.

DOI:10.1016/j.clinbiochem.2011.06.983
PMID:21763300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3177995/
Abstract

OBJECTIVE

The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.

DESIGN AND METHODS

A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.

RESULTS

Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.

CONCLUSIONS

The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.

摘要

目的

开发一种适用于检测多种生物标志物的细胞因子检测分析方法，以提高分枝杆菌病的诊断水平。

设计与方法

利用上转换磷（UCP）报告物技术开发了侧向流动（LF）检测分析方法来检测白细胞介素-10（IL-10）。使用麻风病患者的血液样本对该分析方法进行了评估。探索了针对以下目标的多重应用：1）在检测缓冲液中检测 IL-10 和干扰素-γ（IFN-γ）；2）在麻风病患者血清中检测 IL-10 和抗酚性糖脂（PGL-I）抗体。

结果

在血清中检测到低于目标水平 100pg/mL 的 IL-10。与 ELISA 的比较显示具有定量相关性，R²值为 0.92。已证明细胞因子的多重化以及细胞因子和抗体的同时检测。

结论

UCP-LF IL-10 分析方法是 IL-10 ELISA 的一种用户友好、快速的替代方法，适用于不同细胞因子的多重检测，并且可以与抗体检测分析方法合并，以同时检测细胞和体液免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/93ba96172410/nihms310566f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/a17e4288d436/nihms310566f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/8f49331b35f1/nihms310566f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/d69701e800f4/nihms310566f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/71be44a68374/nihms310566f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/652cd2d731d9/nihms310566f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/93ba96172410/nihms310566f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/a17e4288d436/nihms310566f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/8f49331b35f1/nihms310566f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/d69701e800f4/nihms310566f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/71be44a68374/nihms310566f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/652cd2d731d9/nihms310566f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46b4/3177995/93ba96172410/nihms310566f6.jpg

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