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侧向流动检测法同时检测细胞免疫和体液免疫应答。

Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.

机构信息

Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.

出版信息

Clin Biochem. 2011 Oct;44(14-15):1241-6. doi: 10.1016/j.clinbiochem.2011.06.983. Epub 2011 Jul 6.

DOI:10.1016/j.clinbiochem.2011.06.983
PMID:21763300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3177995/
Abstract

OBJECTIVE

The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.

DESIGN AND METHODS

A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.

RESULTS

Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.

CONCLUSIONS

The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.

摘要

目的

开发一种适用于检测多种生物标志物的细胞因子检测分析方法，以提高分枝杆菌病的诊断水平。

设计与方法

利用上转换磷（UCP）报告物技术开发了侧向流动（LF）检测分析方法来检测白细胞介素-10（IL-10）。使用麻风病患者的血液样本对该分析方法进行了评估。探索了针对以下目标的多重应用：1）在检测缓冲液中检测 IL-10 和干扰素-γ（IFN-γ）；2）在麻风病患者血清中检测 IL-10 和抗酚性糖脂（PGL-I）抗体。

结果

在血清中检测到低于目标水平 100pg/mL 的 IL-10。与 ELISA 的比较显示具有定量相关性，R²值为 0.92。已证明细胞因子的多重化以及细胞因子和抗体的同时检测。

结论

UCP-LF IL-10 分析方法是 IL-10 ELISA 的一种用户友好、快速的替代方法，适用于不同细胞因子的多重检测，并且可以与抗体检测分析方法合并，以同时检测细胞和体液免疫。

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本文引用的文献

1
Rapid quantitative detection of by lateral-flow immunoassay and up-converting phosphor technology-based biosensor.
Sens Actuators B Chem. 2006 Dec 7;119(2):656-663. doi: 10.1016/j.snb.2006.01.029. Epub 2006 Feb 17.
2
Host markers in QuantiFERON supernatants differentiate active TB from latent TB infection: preliminary report.
BMC Pulm Med. 2009 May 16;9:21. doi: 10.1186/1471-2466-9-21.
3
Relationship between circulating levels of IFN-gamma, IL-10, CXCL9 and CCL2 in pulmonary and extrapulmonary tuberculosis is dependent on disease severity.
Scand J Immunol. 2009 Mar;69(3):259-67. doi: 10.1111/j.1365-3083.2008.02217.x.
4
Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody.
Diagn Microbiol Infect Dis. 2009 Feb;63(2):165-72. doi: 10.1016/j.diagmicrobio.2008.10.020.
5
Genetic, epidemiological and biological analysis of interleukin-10 promoter single-nucleotide polymorphisms suggests a definitive role for -819C/T in leprosy susceptibility.
Genes Immun. 2009 Mar;10(2):174-80. doi: 10.1038/gene.2008.97. Epub 2008 Dec 25.
6
Human CD4 and CD8 regulatory T cells in infectious diseases and vaccination.
Hum Immunol. 2008 Nov;69(11):760-70. doi: 10.1016/j.humimm.2008.07.017. Epub 2008 Oct 7.
7
A user-friendly, highly sensitive assay to detect the IFN-gamma secretion by T cells.
Clin Biochem. 2008 Apr;41(6):440-4. doi: 10.1016/j.clinbiochem.2007.12.015. Epub 2008 Jan 3.
8
Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum.
J Clin Microbiol. 2008 Jan;46(1):171-6. doi: 10.1128/JCM.00877-07. Epub 2007 Oct 17.
9
Interferon gamma/IL10 ratio defines the disease severity in pulmonary and extra pulmonary tuberculosis.
Tuberculosis (Edinb). 2007 Jul;87(4):279-87. doi: 10.1016/j.tube.2007.03.004. Epub 2007 May 29.
10
Evaluation of UPlink-RSV: prototype rapid antigen test for detection of respiratory syncytial virus infection.
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