侧向流动检测法同时检测细胞免疫和体液免疫应答。
Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
机构信息
Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
出版信息
Clin Biochem. 2011 Oct;44(14-15):1241-6. doi: 10.1016/j.clinbiochem.2011.06.983. Epub 2011 Jul 6.
Abstract
OBJECTIVE
The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.
DESIGN AND METHODS
A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.
RESULTS
Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.
CONCLUSIONS
The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
摘要
目的
开发一种适用于检测多种生物标志物的细胞因子检测分析方法，以提高分枝杆菌病的诊断水平。
设计与方法
利用上转换磷（UCP）报告物技术开发了侧向流动（LF）检测分析方法来检测白细胞介素-10（IL-10）。使用麻风病患者的血液样本对该分析方法进行了评估。探索了针对以下目标的多重应用：1）在检测缓冲液中检测 IL-10 和干扰素-γ（IFN-γ）；2）在麻风病患者血清中检测 IL-10 和抗酚性糖脂（PGL-I）抗体。
结果
在血清中检测到低于目标水平 100pg/mL 的 IL-10。与 ELISA 的比较显示具有定量相关性，R²值为 0.92。已证明细胞因子的多重化以及细胞因子和抗体的同时检测。
结论
UCP-LF IL-10 分析方法是 IL-10 ELISA 的一种用户友好、快速的替代方法，适用于不同细胞因子的多重检测，并且可以与抗体检测分析方法合并，以同时检测细胞和体液免疫。
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本文引用的文献
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Sens Actuators B Chem. 2006 Dec 7;119(2):656-663. doi: 10.1016/j.snb.2006.01.029. Epub 2006 Feb 17.
2
Host markers in QuantiFERON supernatants differentiate active TB from latent TB infection: preliminary report.全血γ-干扰素释放试验上清液中的宿主标志物可区分活动性结核病与潜伏性结核感染：初步报告
BMC Pulm Med. 2009 May 16;9:21. doi: 10.1186/1471-2466-9-21.
3
Relationship between circulating levels of IFN-gamma, IL-10, CXCL9 and CCL2 in pulmonary and extrapulmonary tuberculosis is dependent on disease severity.肺结核和肺外结核患者体内干扰素-γ、白细胞介素-10、CXCL9和CCL2的循环水平之间的关系取决于疾病的严重程度。
Scand J Immunol. 2009 Mar;69(3):259-67. doi: 10.1111/j.1365-3083.2008.02217.x.
4
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5
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Genes Immun. 2009 Mar;10(2):174-80. doi: 10.1038/gene.2008.97. Epub 2008 Dec 25.
6
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7
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8
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