锌纳米颗粒可改善雄性大鼠大脑中由银纳米颗粒诱导的氧化应激和细胞凋亡。

Zinc nanoparticles ameliorate oxidative stress and apoptosis induced by silver nanoparticles in the brain of male rats.

作者信息

Noshy Peter A, Yasin Noha A E, Rashad Maha M, Shehata Asmaa M, Salem Fatma M S, El-Saied Eiman M, Mahmoud Mohamed Y

机构信息

Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

出版信息

Neurotoxicology. 2023 Mar;95:193-204. doi: 10.1016/j.neuro.2023.02.005. Epub 2023 Feb 14.

DOI:10.1016/j.neuro.2023.02.005

PMID:36796650

Abstract

The current study was conducted to investigate the possible ameliorative role of zinc nanoparticles (Zn NPs) against silver nanoparticles (Ag NPs)-induced oxidative and apoptotic brain damage in adult male rats. Twenty-four mature Wistar rats were randomly and equally divided into four groups: control group, Ag NPs group, Zn NPs group, and Ag NPs + Zn NPs group. Rats were exposed to Ag NPs (50 mg/kg) and/or Zn NPs (30 mg/kg) daily by oral gavage for 12 weeks. The results revealed that exposure to Ag NPs significantly increased malondialdehyde (MDA) content, decreased catalase and reduced glutathione (GSH) activities, downregulated the relative mRNA expression of antioxidant-related genes (Nrf-2 and SOD), and upregulated the relative mRNA expression of apoptosis-related genes (Bax, caspase 3 and caspase 9) in the brain tissue. Furthermore, severe neuropathological lesions with a substantial increase in the caspase 3 and glial fibrillary acidic protein (GFAP) immunoreactivity were observed in the cerebrum and cerebellum of Ag NPs-exposed rats. Conversely, co-administration of Zn NPs with Ag NPs significantly ameliorated most of these neurotoxic effects. Collectively, Zn NPs can be used as a potent prophylactic agent against Ag NPs-induced oxidative and apoptotic neural damage.

摘要

本研究旨在探讨锌纳米颗粒（Zn NPs）对成年雄性大鼠银纳米颗粒（Ag NPs）诱导的氧化和凋亡性脑损伤可能的改善作用。将24只成熟的Wistar大鼠随机等分为四组：对照组、Ag NPs组、Zn NPs组和Ag NPs + Zn NPs组。通过口服灌胃法，大鼠每天暴露于Ag NPs（50 mg/kg）和/或Zn NPs（30 mg/kg），持续12周。结果显示，暴露于Ag NPs显著增加了脑组织中丙二醛（MDA）含量，降低了过氧化氢酶和还原型谷胱甘肽（GSH）活性，下调了抗氧化相关基因（Nrf-2和SOD）的相对mRNA表达，并上调了凋亡相关基因（Bax、caspase 3和caspase 9）的相对mRNA表达。此外，在暴露于Ag NPs的大鼠的大脑和小脑中观察到严重的神经病理损伤，caspase 3和胶质纤维酸性蛋白（GFAP）免疫反应性显著增加。相反，Zn NPs与Ag NPs联合给药显著改善了大多数这些神经毒性作用。总体而言，Zn NPs可作为一种有效的预防剂，对抗Ag NPs诱导的氧化和凋亡性神经损伤。