Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Biol Trace Elem Res. 2022 Mar;200(3):1201-1211. doi: 10.1007/s12011-021-02713-2. Epub 2021 Apr 14.
Silver nanoparticles (Ag-NPs) have various pharmaceutical and biomedical applications owing to their unique physicochemical properties. Zinc (Zn) is an essential trace element, a strong antioxidant, and has a primary role in gene expression, enzymatic reactions, and protein synthesis. The present study aims to explore the toxic effects of Ag-NPs (50 nm) on the liver and kidney of rats and also to evaluate the potential protective effect of Zn-NPs (100 nm) against these adverse effects. Forty adult Sprague-Dawley rats were randomly divided into four equal groups: control group, Ag-NPs group, Zn-NPs group, and Ag-NPs + Zn-NPs group. Ag-NPs (50 mg/kg) and/or Zn-NPs (30 mg/kg) were administered daily by gavage for 90 days. The results showed that exposure to Ag-NPs increased serum ALT, AST, urea, and creatinine. Ag-NPs also induced oxidative stress and lipid peroxidation and increased inflammatory cytokines in hepatic and renal tissues. Moreover, histopathological and immunohistochemical examinations revealed various histological alterations and positive caspase-3 expressions in the liver and kidney following exposure to Ag-NPs. On the other hand, most of these toxic effects were ameliorated by co-administration of Zn-NPs. It was concluded that Ag-NPs have hepatotoxic and nephrotoxic effects in rats via different mechanisms including oxidative stress, inflammation, and apoptosis and that Zn-NPs can be used to alleviate these harmful effects by their antioxidative, anti-inflammatory, and antiapoptotic properties.
纳米银颗粒（Ag-NPs）由于其独特的物理化学性质，具有各种药物和生物医学应用。锌（Zn）是一种必需的微量元素，具有强大的抗氧化作用，在基因表达、酶反应和蛋白质合成中起主要作用。本研究旨在探讨纳米银颗粒（50nm）对大鼠肝脏和肾脏的毒性作用，并评估纳米锌颗粒（100nm）对这些不良反应的潜在保护作用。40 只成年 Sprague-Dawley 大鼠随机分为四组：对照组、Ag-NPs 组、Zn-NPs 组和 Ag-NPs+Zn-NPs 组。Ag-NPs（50mg/kg）和/或 Zn-NPs（30mg/kg）通过灌胃每天给药，共 90 天。结果表明，暴露于 Ag-NPs 会增加血清 ALT、AST、尿素和肌酐。Ag-NPs 还诱导氧化应激和脂质过氧化，并增加肝和肾组织中的炎症细胞因子。此外，组织病理学和免疫组织化学检查显示，暴露于 Ag-NPs 后，肝脏和肾脏出现各种组织学改变和 caspase-3 阳性表达。另一方面，Zn-NPs 的共同给药改善了大部分这些毒性作用。结论是，Ag-NPs 通过不同的机制（包括氧化应激、炎症和细胞凋亡）对大鼠具有肝毒性和肾毒性，而 Zn-NPs 可以通过其抗氧化、抗炎和抗凋亡特性来减轻这些有害影响。
