College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, PR China.
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, PR China.
J Therm Biol. 2023 Feb;112:103461. doi: 10.1016/j.jtherbio.2023.103461. Epub 2023 Jan 14.
In this study, the model of heat stress was constructed in primary chick embryonic myocardial cells at 42 °C for 4 h. Proteome analysis using DIA identified 245 differentially expressed proteins (DEPs) (Q-value <0.05, fold change >1.5), of which 63 proteins were up-regulated and 182 proteins were down-regulated. Many were related to metabolism, oxidative stress, oxidative phosphorylation and apoptosis. Gene Ontology (GO) analysis showed that many DEPs under heat stress were involved in regulating metabolites and energy, cellular respiration, catalytic activity and stimulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEPs were enriched in metabolic pathways, oxidative phosphorylation, citrate cycle (TCA cycle), cardiac muscle contraction, and carbon metabolism. The results could help understanding of the effect of heat stress on myocardial cells and even the heart and possible action mechanism at the protein level.
在这项研究中，将 42°C 下孵育 4 小时的原代鸡胚心肌细胞构建为热应激模型。使用 DIA 进行蛋白质组分析鉴定出 245 个差异表达蛋白（DEPs）（Q 值<0.05，倍数变化>1.5），其中 63 个蛋白上调，182 个蛋白下调。许多与代谢、氧化应激、氧化磷酸化和细胞凋亡有关。GO 分析表明，热应激下的许多 DEPs 参与调节代谢物和能量、细胞呼吸、催化活性和刺激。KEGG 分析表明，DEPs 富集在代谢途径、氧化磷酸化、柠檬酸循环（TCA 循环）、心肌收缩和碳代谢中。这些结果有助于了解热应激对心肌细胞甚至心脏的影响及其在蛋白质水平上的可能作用机制。
