Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China.
J Coll Physicians Surg Pak. 2023 Feb;33(2):149-152. doi: 10.29271/jcpsp.2023.02.149.
To determine the function of the YAP-JNK-mitophagy signalling pathway in gastric cancer (GC).
An observational study.
Seventh People's Hospital of Shanghai University of TCM (Traditional Chinese Medicine), between June 2019 and June 2021.
Tissues from 30 cases of gastric cancer and corresponding adjacent tissues were collected. RT-qPCR was employed to detect the expression of YAP and JNK in GC samples. MTT, Wound healing and Transwell assays were used to detect changes in GC cell proliferation, migration, and invasion under different stimulation. LC3 immunofluorescence and mitochondrial membrane potential detection were used to analyse the occurrence of mitochondrial autophagy.
The expression of YAP and JNK were significantly increased in GC tissues (p=0.024, 0.033). YAP knockdown inhibited GC cell proliferation, migration, and invasion. Further studies showed that YAP affects GC cell function by targeting JNK. In addition, YAP-JNK signalling was found to regulate GC cell proliferation, migration, and invasion mainly through regulating the occurrence of mitophagy.
These findings revealed that YAP-JNK promotes the development of GC by targeting mitophagy.
Gastric cancer, YAP, JNK, Autophagy.
研究 YAP-JNK-自噬信号通路在胃癌（GC）中的作用。
观察性研究。
上海中医药大学第七人民医院，2019 年 6 月至 2021 年 6 月。
收集 30 例胃癌组织及相应的癌旁组织。采用 RT-qPCR 检测 GC 样本中 YAP 和 JNK 的表达。MTT、划痕愈合和 Transwell 检测在不同刺激下 GC 细胞增殖、迁移和侵袭的变化。LC3 免疫荧光和线粒体膜电位检测分析线粒体自噬的发生。
GC 组织中 YAP 和 JNK 的表达明显增加（p=0.024，0.033）。YAP 敲低抑制 GC 细胞增殖、迁移和侵袭。进一步的研究表明，YAP 通过靶向 JNK 影响 GC 细胞功能。此外，YAP-JNK 信号通路主要通过调节自噬的发生来调节 GC 细胞的增殖、迁移和侵袭。
这些发现表明 YAP-JNK 通过靶向自噬促进 GC 的发展。
胃癌、YAP、JNK、自噬。
