Richards T L, Davis C A, Barker B R, Beinert W D, Genant H K
Department of Radiology, University of California, San Francisco.
Invest Radiol. 1987 Sep;22(9):741-6. doi: 10.1097/00004424-198709000-00008.
Proton NMR spectroscopy (spatially resolved) at 0.7 Tesla with a whole body superconducting magnet was performed on bone marrow from human lumbar spine to measure the lipid/water ratio. Phase-encoded proton spectroscopy was performed by using (1) a spin-echo sequence with selective 90 degrees and 180 degrees rf pulses on the Z and X axes; (2) 64 different phase encodes along the Y axis; and (3) echo acquisition with no gradient. The lipid/water ratios also were measured by the Folch chemical extraction technique. The set of phase-encoded spectra obtained from the spine demonstrates the chemical difference between the vertebral disk (large water peak and no lipid peak) and the marrow (smaller water peak and detectable lipids). Lipid/water ratios were lower than the chemically measured ratios. The phase-encoded proton spectroscopic technique presented in this study allowed rapid acquisition of localized spectra from the bone marrow in each of three vertebral bodies of the intact human spine. This technique provided a measure of the mobile lipid/water ratio weighted by relaxation times.
使用0.7特斯拉的全身超导磁体对人腰椎骨髓进行了（空间分辨的）质子核磁共振光谱分析，以测量脂质/水比率。通过以下方式进行相位编码质子光谱分析：（1）在Z轴和X轴上使用具有选择性90度和180度射频脉冲的自旋回波序列；（2）沿Y轴进行64种不同的相位编码；（3）无梯度回波采集。脂质/水比率也通过Folch化学提取技术进行测量。从脊柱获得的一组相位编码光谱显示了椎间盘（大水峰且无脂质峰）和骨髓（较小水峰且可检测到脂质）之间的化学差异。脂质/水比率低于化学测量比率。本研究中提出的相位编码质子光谱技术能够快速从完整人脊柱的三个椎体中的每个椎体的骨髓中获取局部光谱。该技术提供了一种由弛豫时间加权的可移动脂质/水比率的测量方法。
