Liao Jiakai, Deng Ban, Yang Qixin, Li Yu, Zhang Yuxiang, Cong Jiajing, Wang Xiaqin, Kohnen Markus V, Liu Zhong-Jian, Lu Meng-Zhu, Lin Deshu, Gu Lianfeng, Liu Bobin
Basic Forestry and Proteomics Research Center, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, China.
New Phytol. 2023 May;238(4):1479-1497. doi: 10.1111/nph.18815. Epub 2023 Mar 8.
The acquisition of dormancy capabilities has enabled plants to survive in adverse terrestrial environmental conditions. Dormancy accumulation and release is coupled with light signaling, which is well studied in Arabidopsis, but it is unclear in the distant nonvascular relative. We study the characteristics and function on dormancy regulation of a blue light receptor cryptochrome in Marchantia polymorpha (MpCRY). Here, we identified MpCRY via bioinformatics and mutant complement analysis. The biochemical characteristics were assessed by multiple protein-binding assays. The function of MpCRY in gemma dormancy was clarified by overexpression and mutation of MpCRY, and its mechanism was analyzed via RNA sequencing and quantitative PCR analyses associated with hormone treatment. We found that the unique MpCRY protein in M. polymorpha undergoes both blue light-promoted interaction with itself (self-interaction) and blue light-dependent phosphorylation. MpCRY has the specific characteristics of blue light-induced nuclear localization and degradation. We further demonstrated that MpCRY transcriptionally represses abscisic acid (ABA) signaling-related gene expression to suppress gemma dormancy, which is dependent on blue light signaling. Our findings indicate that MpCRY possesses specific biochemical and molecular characteristics, and modulates ABA signaling under blue light conditions to regulate gemma dormancy in M. polymorpha.
获得休眠能力使植物能够在恶劣的陆地环境条件下生存。休眠的积累和解除与光信号传导相关，这在拟南芥中已有深入研究，但在亲缘关系较远的非维管植物中尚不清楚。我们研究了多形苔（MpCRY）中蓝光受体隐花色素在休眠调控方面的特征和功能。在这里，我们通过生物信息学和突变体互补分析鉴定了MpCRY。通过多种蛋白质结合试验评估其生化特性。通过MpCRY的过表达和突变阐明了MpCRY在芽孢休眠中的功能，并通过与激素处理相关的RNA测序和定量PCR分析其机制。我们发现多形苔中独特的MpCRY蛋白既经历蓝光促进的自身相互作用（自相互作用），又经历蓝光依赖的磷酸化。MpCRY具有蓝光诱导的核定位和降解的特定特征。我们进一步证明，MpCRY转录抑制脱落酸（ABA）信号相关基因的表达以抑制芽孢休眠，这依赖于蓝光信号传导。我们的研究结果表明，MpCRY具有特定的生化和分子特征，并在蓝光条件下调节ABA信号传导以调控多形苔的芽孢休眠。
