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蓝光下 MpCRY 介导的 COP1/SPA E3 泛素连接酶在苔藓中的功能。

Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort under Blue Light.

机构信息

Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, Changchun 130062, China.

Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China.

出版信息

Int J Mol Sci. 2021 Dec 23;23(1):158. doi: 10.3390/ijms23010158.

DOI:10.3390/ijms23010158
PMID:35008588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8745113/
Abstract

COP1/SPA1 complex in inhibits photomorphogenesis through the ubiquitination of multiple photo-responsive transcription factors in darkness, but such inhibiting function of COP1/SPA1 complex would be suppressed by cryptochromes in blue light. Extensive studies have been conducted on these mechanisms in whereas little attention has been focused on whether another branch of land plants bryophyte utilizes this blue-light regulatory pathway. To study this problem, we conducted a study in the liverwort and obtained a MpSPA knock-out mutant, in which Mp exhibits the phenotype of an increased percentage of individuals with asymmetrical thallus growth, similar to MpCRY knock-out mutant. We also verified interactions of MpSPA with MpCRY (in a blue light-independent way) and with MpCOP1. Concomitantly, both MpSPA and MpCOP1 could interact with MpHY5, and MpSPA can promote MpCOP1 to ubiquitinate MpHY5 but MpCRY does not regulate the ubiquitination of MpHY5 by MpCOP1/MpSPA complex. These data suggest that COP1/SPA ubiquitinating HY5 is conserved in , but dissimilar to CRY in , MpCRY is not an inhibitor of this process under blue light.

摘要

COP1/SPA1 复合物在黑暗中通过泛素化多个光响应转录因子来抑制光形态发生，但这种 COP1/SPA1 复合物的抑制功能会被蓝光中的隐花色素抑制。人们对这些机制在拟南芥中的作用进行了广泛的研究，而对另一个陆地植物分支苔藓植物是否利用这种蓝光调控途径则关注较少。为了研究这个问题，我们在叶苔中进行了一项研究，获得了一个 MpSPA 敲除突变体，该突变体中 Mp 表现出不对称叶状体生长个体比例增加的表型，类似于 MpCRY 敲除突变体。我们还验证了 MpSPA 与 MpCRY（以非蓝光依赖的方式）和 MpCOP1 的相互作用。同时，MpSPA 和 MpCOP1 都可以与 MpHY5 相互作用，并且 MpSPA 可以促进 MpCOP1 泛素化 MpHY5，但 MpCRY 不调节 MpCOP1/MpSPA 复合物对 MpHY5 的泛素化。这些数据表明，COP1/SPA 泛素化 HY5 在叶苔中是保守的，但与 CRY 不同，在蓝光下，MpCRY 不是这个过程的抑制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/29596ea2b990/ijms-23-00158-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/fb987ef23842/ijms-23-00158-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/8aadac35657d/ijms-23-00158-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/78eb9124933d/ijms-23-00158-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/5315b765299c/ijms-23-00158-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/29596ea2b990/ijms-23-00158-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/fb987ef23842/ijms-23-00158-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/8aadac35657d/ijms-23-00158-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/78eb9124933d/ijms-23-00158-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/5315b765299c/ijms-23-00158-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/873d/8745113/08b14a78fd91/ijms-23-00158-g005.jpg
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