Eichler Katharina, Hampel Stefanie, Alejandro-García Adrián, Calle-Schuler Steven A, Santana-Cruz Alexis, Kmecova Lucia, Blagburn Jonathan M, Hoopfer Eric D, Seeds Andrew M
Institute of Neurobiology, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.
Contributed equally.
bioRxiv. 2023 Dec 15:2023.02.11.528119. doi: 10.1101/2023.02.11.528119.
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements to complex movement sequences. How mechanosensory neurons and their postsynaptic circuits influence such diverse behaviors remains unclear. We previously discovered that perform a body location-prioritized grooming sequence when mechanosensory neurons at different locations on the head and body are simultaneously stimulated by dust (Hampel et al., 2017; Seeds et al., 2014). Here, we identify nearly all mechanosensory neurons on the head that individually elicit aimed grooming of specific head locations, while collectively eliciting a whole head grooming sequence. Different tracing methods were used to reconstruct the projections of these neurons from different locations on the head to their distinct arborizations in the brain. This provides the first synaptic resolution somatotopic map of a head, and defines the parallel-projecting mechanosensory pathways that elicit head grooming.
分布于身体表面的机械感觉神经元对触觉刺激做出反应，并引发多种行为反应，从相对简单的针对刺激位置的运动到复杂的运动序列。机械感觉神经元及其突触后回路如何影响这些多样的行为仍不清楚。我们之前发现，当头部和身体不同位置的机械感觉神经元同时受到灰尘刺激时，[具体主体]会执行身体位置优先的梳理序列（汉佩尔等人，2017年；西兹等人，2014年）。在这里，我们识别出了头部几乎所有的机械感觉神经元，它们单独引发特定头部位置的定向梳理，同时共同引发整个头部的梳理序列。使用了不同的追踪方法来重建这些神经元从头部不同位置到其在大脑中不同分支的投射。这提供了首个头部的突触分辨率躯体定位图，并定义了引发头部梳理的平行投射机械感觉通路。
