Chen Yan, Zheng Bin, Li Haidong, Lin Li, Fan Yuanyuan, Wu Mengai
National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
J Ophthalmol. 2023 Feb 7;2023:3987281. doi: 10.1155/2023/3987281. eCollection 2023.
To investigate the relationship between aqueous humor cytokines and structural characteristics based on optical coherence tomography (OCT) in patients with diabetic macular edema (DME).
Forty eyes of 28 patients with DME diagnosed in the Affiliated Eye Hospital of Wenzhou Medical University at Hangzhou were included. All patients collected aqueous humor during anti-VEGF treatment, and the IL-6, IL-8, IL-10, VEGF, VCAM-1, ICAM-1, TGF-1, FGF, and MCP-1 concentrations were detected. OCT examination was performed before anti-VEGF treatment and 1 month after anti-VEGF operation. Central macular thickness (CMT), macular volume (MV), choroidal thickness (CT), and the number of hyperreflective foci (HRF) were obtained for analysis. Each eye was determined whether there is subretinal effusion (SRD), cystoid macular edema (CME), and diffuse retinal thickening (DRT).
The levels of IL-6 and FGF in DME patients with SRD were significantly higher than those without SRD (all < 0.05). The level of VEGF in DME patients with CME was significantly higher than that in DME patients without CME ( = 0.005); IL-6, TGF-1, and MCP-1 were significantly higher in DME patients with DRT than that without DRT (all < 0.05). There was no significant correlation between aqueous humor cytokines and retinal thickness and retinal volume. However, the thinner the CT, the higher the level of aqueous humor cytokines IL-6 ( = -0.313, = 0.049) and FGF ( = -0.361, = 0.022). A multivariate linear regression analysis showed that IL-6 was significantly correlated with CT ( = 0.002) and SRD ( = 0.017), FGF was also significantly correlated with CT ( = 0.002) and SRD ( = 0.005), and TGF-1 was correlated with triglycerides ( = 0.030) and HRF ( = 0.021).
DME patients with significant macular cystoid edema changes may be related to high VEGF concentrations and thin CT; meanwhile, the presence of SRD or a high number of HRF on OCT macular scans in DME patients may indicate high levels of intraocular inflammatory factors. Thus, OCT morphology characteristics to some extent reflect intraocular inflammatory factors and VEGF levels and may guide treatment alternatives.
基于光学相干断层扫描（OCT）研究糖尿病性黄斑水肿（DME）患者房水细胞因子与结构特征之间的关系。
纳入温州医科大学附属眼视光医院杭州院区确诊的28例DME患者的40只眼。所有患者在抗VEGF治疗期间采集房水，检测IL-6、IL-8、IL-10、VEGF、VCAM-1、ICAM-1、TGF-1、FGF和MCP-1浓度。在抗VEGF治疗前及抗VEGF手术1个月后进行OCT检查。获取中心黄斑厚度（CMT）、黄斑体积（MV）、脉络膜厚度（CT）和高反射灶（HRF）数量进行分析。判断每只眼是否存在视网膜下积液（SRD）、黄斑囊样水肿（CME）和弥漫性视网膜增厚（DRT）。
伴有SRD的DME患者IL-6和FGF水平显著高于无SRD的患者（均P<0.05）。伴有CME的DME患者VEGF水平显著高于无CME的DME患者（P = 0.005）；伴有DRT的DME患者IL-6、TGF-1和MCP-1显著高于无DRT的患者（均P<0.05）。房水细胞因子与视网膜厚度和视网膜体积之间无显著相关性。然而，CT越薄，房水细胞因子IL-6（r = -0.313，P = 0.049）和FGF（r = -0.361，P = 0.022）水平越高。多因素线性回归分析显示，IL-
